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Micromachines 2017, 8(3), 85; doi:10.3390/mi8030085

Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device

1
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
2
Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge CB2 1TN, UK
3
Laser Biomedical Research Center (LBRC), Massachusetts Institute of Technology, Cambridge, MA 02139, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Shih-Chi Chen and Wei-Chuan Shih
Received: 17 January 2017 / Revised: 27 February 2017 / Accepted: 6 March 2017 / Published: 9 March 2017
(This article belongs to the Special Issue MEMS/NEMS for Biomedical Imaging and Sensing)
View Full-Text   |   Download PDF [2422 KB, uploaded 9 March 2017]   |  

Abstract

Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice. View Full-Text
Keywords: multiphoton microscopy; temporal focusing; digital micromirror device multiphoton microscopy; temporal focusing; digital micromirror device
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Park, J.K.; Rowlands, C.J.; So, P.T.C. Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device. Micromachines 2017, 8, 85.

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