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Micromachines 2017, 8(1), 24; doi:10.3390/mi8010024

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

1
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China
2
Chongqing Engineering Research Center of Medical Electronics Technology (Chongqing University), Bioengineering College, Chongqing University, Chongqing 400030, China
3
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
4
College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
5
Chongqing Jinshan Science & Technology (Group) Co., Ltd., Chongqing 401120, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Xiangchun Xuan and Shizhi Qian
Received: 23 September 2016 / Revised: 7 January 2017 / Accepted: 9 January 2017 / Published: 16 January 2017
(This article belongs to the Special Issue Micro/Nano-Chip Electrokinetics)
View Full-Text   |   Download PDF [14151 KB, uploaded 16 January 2017]   |  

Abstract

A giant unilamellar vesicle (GUV), with similar properties to cellular membrane, has been widely studied. Electroformation with its simplicity and accessibility has become the most common method for GUV production. In this work, GUV electroformation in devices with traditional 3D and new 2D electrode structures were studied with respect to the applied electric field. An optimal frequency (10 kHz in the 3D and 1 kHz in the 2D systems) was found in each system. A positive correlation was found between GUV formation and applied voltage in the 3D electrode system from 1 to 10 V. In the 2D electrode system, the yield of the generated GUV increased first but decreased later as voltage increased. These phenomena were further confirmed by numerically calculating the load that the lipid film experienced from the generated electroosmotic flow (EOF). The discrepancy between the experimental and numerical results of the 3D electrode system may be because the parameters that were adopted in the simulations are quite different from those of the lipid film in experiments. The lipid film was not involved in the simulation of the 2D system, and the numerical results matched well with the experiments. View Full-Text
Keywords: electroformation; yield; monodispersity; lipid film electroformation; yield; monodispersity; lipid film
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Wang, Q.; Zhang, X.; Fan, T.; Yang, Z.; Chen, X.; Wang, Z.; Xu, J.; Li, Y.; Hu, N.; Yang, J. Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems. Micromachines 2017, 8, 24.

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