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Micromachines 2017, 8(1), 10; doi:10.3390/mi8010010

Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel

1
School of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Korea
2
Department of Polymer Science, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Korea
3
Boditech Med Inc., 43, Geodudanji 1-gil, Dongnae-myeon, Chuncheon-si, Gang-won-do 24398, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Xunli Zhang, Dario Carugo and Nam-Trung Nguyen
Received: 11 November 2016 / Revised: 21 December 2016 / Accepted: 30 December 2016 / Published: 3 January 2017
(This article belongs to the Special Issue Microfluidic Technologies for Drug Delivery)
View Full-Text   |   Download PDF [8183 KB, uploaded 3 January 2017]   |  

Abstract

This paper challenges the production of the protein nanoparticles using the conjugation of Ni2+ complexed nitrilotriacetic acid end-functionalized polystyrene (Ni-NTA-PS) and histidine tagged GFP (His-GFP) hybrid. The microfluidic synthesis of the protein nanoparticle with the advantages of a uniform size, a fast reaction, and a precise control of preparation conditions is examined. The self-assembly occurs on the interfacial surface of the multi-laminated laminar flow stably formed in the microchannel. The clogging of the produced protein nanoparticles on the channel surface is solved by adding a retarding inlet channel. The size and shape of the produced protein nanoparticles are measured by the analysis of transmission electron microscopy (TEM) and scanning electron microscope (SEM) images, and the attachment of the protein is visualized with a green fluorescent image. Future research includes the encapsulation of vaccines and the coating of antigens on the protein surface. View Full-Text
Keywords: protein nanoparticles; microfluidic synthesis; self-assembly; lamination flow; protein-polymer hybrid protein nanoparticles; microfluidic synthesis; self-assembly; lamination flow; protein-polymer hybrid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jeon, H.J.; Lee, C.Y.; Kim, M.J.; Nguyen, X.D.; Park, D.H.; Kim, H.H.; Go, J.S.; Paik, H.-J. Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel. Micromachines 2017, 8, 10.

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