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Micromachines 2016, 7(5), 77; doi:10.3390/mi7050077

Magnetic Particle Plug-Based Assays for Biomarker Analysis

Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, UK
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Author to whom correspondence should be addressed.
Academic Editors: Manabu Tokeshi and Kiichi Sato
Received: 13 February 2016 / Revised: 1 April 2016 / Accepted: 13 April 2016 / Published: 26 April 2016
(This article belongs to the Special Issue Micro/Nano Devices for Chemical Analysis)
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Abstract

Conventional immunoassays offer selective and quantitative detection of a number of biomarkers, but are laborious and time-consuming. Magnetic particle-based assays allow easy and rapid selection of analytes, but still suffer from the requirement of tedious multiple reaction and washing steps. Here, we demonstrate the trapping of functionalised magnetic particles within a microchannel for performing rapid immunoassays by flushing consecutive reagent and washing solutions over the trapped particle plug. Three main studies were performed to investigate the potential of the platform for quantitative analysis of biomarkers: (i) a streptavidin-biotin binding assay; (ii) a sandwich assay of the inflammation biomarker, C-reactive protein (CRP); and (iii) detection of the steroid hormone, progesterone (P4), towards a competitive assay. Quantitative analysis with low limits of detection was demonstrated with streptavidin-biotin, while the CRP and P4 assays exhibited the ability to detect clinically relevant analytes, and all assays were completed in only 15 min. These preliminary results show the great potential of the platform for performing rapid, low volume magnetic particle plug-based assays of a range of clinical biomarkers via an exceedingly simple technique. View Full-Text
Keywords: C-reactive protein (CRP); progesterone (P4); immunoassays; magnetic particles; magnetism; microfluidics; particle trapping C-reactive protein (CRP); progesterone (P4); immunoassays; magnetic particles; magnetism; microfluidics; particle trapping
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Phurimsak, C.; Tarn, M.D.; Pamme, N. Magnetic Particle Plug-Based Assays for Biomarker Analysis. Micromachines 2016, 7, 77.

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