Next Article in Journal
Analysis of the Micromachining Process of Dielectric and Metallic Substrates Immersed in Water with Femtosecond Pulses
Next Article in Special Issue
Two-Layer Microstructures Fabricated by One-Step Anisotropic Wet Etching of Si in KOH Solution
Previous Article in Journal / Special Issue
Liquid Gradient Refractive Index Microlens for Dynamically Adjusting the Beam Focusing
Article Menu

Export Article

Open AccessArticle
Micromachines 2015, 6(12), 1996-2009; doi:10.3390/mi6121470

Fluid Flow Shear Stress Stimulation on a Multiplex Microfluidic Device for Rat Bone Marrow Stromal Cell Differentiation Enhancement

1
Department of Mechanical Engineering, National Central University, 32001 Taoyuan, Taiwan
2
Proteomics Laboratory, Cathay General Hospital, 22174 New Taipei City, Taiwan
3
Institute of Biomedical Engineering, National Central University, 32001 Taoyuan, Taiwan
4
School of Medicine, Fu Jen Catholic University, 24205 New Taipei City, Taiwan
This paper is an extended version of our paper presented in the 5th International Conference on Optofluidics 2015, Taipei, Taiwan, 26–29 July 2015.
*
Author to whom correspondence should be addressed.
Academic Editors: Shih-Kang Fan, Da-Jeng Yao and Yi-Chung Tung
Received: 28 October 2015 / Revised: 25 November 2015 / Accepted: 7 December 2015 / Published: 11 December 2015
(This article belongs to the Special Issue Optofluidics 2015)
View Full-Text   |   Download PDF [5280 KB, uploaded 11 December 2015]   |  

Abstract

Microfluidic devices provide low sample consumption, high throughput, high integration, and good environment controllability advantages. An alternative to conventional bioreactors, microfluidic devices are a simple and effective platform for stem cell investigations. In this study, we describe the design of a microfluidic device as a chemical and mechanical shear stress bioreactor to stimulate rat bone marrow stromal cells (rBMSCs) into neuronal cells. 1-methyl-3-isobutylxanthine (IBMX) was used as a chemical reagent to induce rBMSCs differentiation into neurons. Furthermore, the shear stress applied to rBMSCs was generated by laminar microflow in the microchannel. Four parallel microfluidic chambers were designed to provide a multiplex culture platform, and both the microfluidic chamber-to-chamber, as well as microfluidic device-to-device, culture stability were evaluated. Our research shows that rBMSCs were uniformly cultured in the microfluidic device and differentiated into neuronal cells with IBMX induction. A three-fold increase in the neuronal cell differentiation ratio was noted when rBMSCs were subjected to both IBMX and fluid flow shear stress stimulation. Here, we propose a microfluidic device which is capable of providing chemical and physical stimulation, and could accelerate neuronal cell differentiation from bone marrow stromal cells. View Full-Text
Keywords: microfluidics; rat bone marrow stromal cell; stem cell stimulation; fluid flow shear stress; neuronal cell differentiation microfluidics; rat bone marrow stromal cell; stem cell stimulation; fluid flow shear stress; neuronal cell differentiation
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tsao, C.-W.; Cheng, Y.-C.; Cheng, J.-H. Fluid Flow Shear Stress Stimulation on a Multiplex Microfluidic Device for Rat Bone Marrow Stromal Cell Differentiation Enhancement. Micromachines 2015, 6, 1996-2009.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top