Next Article in Journal
Performance Characterization of Micromachined Inductive Suspensions Based on 3D Wire-Bonded Microcoils
Next Article in Special Issue
Multiplex, Quantitative, Reverse Transcription PCR Detection of Influenza Viruses Using Droplet Microfluidic Technology
Previous Article in Journal
Numerical Investigation of Pileup Process in Metal Microdroplet Deposition Manufacture
Previous Article in Special Issue
Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning
Article Menu

Export Article

Open AccessArticle
Micromachines 2014, 5(4), 1445-1468; doi:10.3390/mi5041445

Analysis of Thermal Performance in a Bidirectional Thermocycler by Including Thermal Contact Characteristics

1
Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 912, Taiwan
2
Department of Mechanical Engineering, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 912, Taiwan
*
Author to whom correspondence should be addressed.
Received: 17 October 2014 / Revised: 21 November 2014 / Accepted: 4 December 2014 / Published: 12 December 2014
(This article belongs to the Special Issue Biomedical Microdevices)
View Full-Text   |   Download PDF [13190 KB, uploaded 12 December 2014]   |  

Abstract

This paper illustrates an application of a technique for predicting the thermal characteristics of a bidirectional thermocycling device for polymerase chain reaction (PCR). The micromilling chamber is oscillated by a servo motor and contacted with different isothermal heating blocks to successfully amplify the DNA templates. Because a comprehensive database of contact resistance factors does not exist, it causes researchers to not take thermal contact resistance into consideration at all. We are motivated to accurately determine the thermal characteristics of the reaction chamber with thermal contact effects existing between the heater surface and the chamber surface. Numerical results show that the thermal contact effects between the heating blocks and the reaction chamber dominate the temperature variations and the ramping rates inside the PCR chamber. However, the influences of various temperatures of the ambient conditions on the sample temperature during three PCR steps can be negligible. The experimental temperature profiles are compared well with the numerical simulations by considering the thermal contact conductance coefficient which is empirical by the experimental fitting. To take thermal contact conductance coefficients into consideration in the thermal simulation is recommended to predict a reasonable temperature profile of the reaction chamber during various thermal cycling processes. Finally, the PCR experiments present that Hygromycin B DNA templates are amplified successfully. Furthermore, our group is the first group to introduce the thermal contact effect into theoretical study that has been applied to the design of a PCR device, and to perform the PCR process in a bidirectional thermocycler. View Full-Text
Keywords: polymerase chain reaction; thermal cycle; thermal contact conductance; ramping rate; bidirectional polymerase chain reaction; thermal cycle; thermal contact conductance; ramping rate; bidirectional
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chen, J.J.; Li, K.T.; Chen, W.H.; Yang, Y.T. Analysis of Thermal Performance in a Bidirectional Thermocycler by Including Thermal Contact Characteristics. Micromachines 2014, 5, 1445-1468.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top