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Micromachines 2014, 5(2), 341-358; doi:10.3390/mi5020341

Optimization of Femtosecond Laser Polymerized Structural Niches to Control Mesenchymal Stromal Cell Fate in Culture

1
LaBS, Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, Milano 20133, Italy
2
Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milano 20133, Italy
3
Center for Nano Science and Technology of IIT@POLIMI, Via Pascoli 70/3, Milano 20133, Italy, Milano 20133, Italy
4
Istituto di Fotonica e Nanotecnologie-Consiglio Nazionale delle Ricerche (IFN-CNR), Piazza Leonardo da Vinci 32, Milano 20133, Italy
*
Author to whom correspondence should be addressed.
Received: 31 March 2014 / Revised: 4 June 2014 / Accepted: 4 June 2014 / Published: 11 June 2014
(This article belongs to the Special Issue Laser Micro- and Nano- Processing)
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Abstract

We applied two-photon polymerization to fabricate 3D synthetic niches arranged in complex patterns to study the effect of mechano-topological parameters on morphology, renewal and differentiation of rat mesenchymal stromal cells. Niches were formed in a photoresist with low auto-fluorescence, which enabled the clear visualization of the fluorescence emission of the markers used for biological diagnostics within the internal niche structure. The niches were structurally stable in culture up to three weeks. At three weeks of expansion in the niches, cell density increased by almost 10-fold and was 67% greater than in monolayer culture. Evidence of lineage commitment was observed in monolayer culture surrounding the structural niches, and within cell aggregates, but not inside the niches. Thus, structural niches were able not only to direct stem cell homing and colony formation, but also to guide aggregate formation, providing increased surface-to-volume ratios and space for stem cells to adhere and renew, respectively. View Full-Text
Keywords: mesenchymal stem cell; niche; scaffold; proliferation; two photon polymerization; photoresist; femtosecond laser mesenchymal stem cell; niche; scaffold; proliferation; two photon polymerization; photoresist; femtosecond laser
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Raimondi, M.T.; Nava, M.M.; Eaton, S.M.; Bernasconi, A.; Vishnubhatla, K.C.; Cerullo, G.; Osellame, R. Optimization of Femtosecond Laser Polymerized Structural Niches to Control Mesenchymal Stromal Cell Fate in Culture. Micromachines 2014, 5, 341-358.

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