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FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation
DTU-Nanotech, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 7, DK-1870, Frederiksberg, Denmark
Wilhelm Johannsen Centre for Functional Genome Research, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark
Kennedy Center, Gl. Landevej, 2600 Glostrup, Denmark
* Author to whom correspondence should be addressed.
Received: 10 February 2011; in revised form: 25 March 2011 / Accepted: 27 March 2011 / Published: 4 April 2011
Abstract: We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3–4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.
Keywords: Fluorescence In Situ Hybridization (FISH); lab on chip; genetic analysis; cytogenetics; chromosome spreading; metaphase FISH; chromosomal translocations
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Shah, P.; Vedarethinam, I.; Kwasny, D.; Andresen, L.; Skov, S.; Silahtaroglu, A.; Tümer, Z.; Dimaki, M.; Svendsen, W.E. FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation. Micromachines 2011, 2, 116-128.
Shah P, Vedarethinam I, Kwasny D, Andresen L, Skov S, Silahtaroglu A, Tümer Z, Dimaki M, Svendsen WE. FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation. Micromachines. 2011; 2(2):116-128.
Shah, Pranjul; Vedarethinam, Indumathi; Kwasny, Dorota; Andresen, Lars; Skov, Søren; Silahtaroglu, Asli; Tümer, Zeynep; Dimaki, Maria; Svendsen, Winnie E. 2011. "FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation." Micromachines 2, no. 2: 116-128.