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• Shah, P
• Vedarethinam, I
• Kwasny, D
• Andresen, L
• Skov, S
• Silahtaroglu, A
• Tümer, Z
• Dimaki, M
• Svendsen, W
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• Shah, P
• Vedarethinam, I
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• Andresen, L
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• Silahtaroglu, A
• Tümer, Z
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• Shah, P
• Vedarethinam, I
• Kwasny, D
• Andresen, L
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Micromachines 2011, 2(2), 116-128; doi:10.3390/mi2020116

Article

FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation

Pranjul Shah ¹ , Indumathi Vedarethinam ¹ , Dorota Kwasny ¹ , Lars Andresen ^1,2 , Søren Skov ² , Asli Silahtaroglu ³ , Zeynep Tümer ⁴ , Maria Dimaki ¹  and Winnie E. Svendsen ^1,*

¹ DTU-Nanotech, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark ² Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 7, DK-1870, Frederiksberg, Denmark ³ Wilhelm Johannsen Centre for Functional Genome Research, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark ⁴ Kennedy Center, Gl. Landevej, 2600 Glostrup, Denmark

* Author to whom correspondence should be addressed.

Received: 10 February 2011; in revised form: 25 March 2011 / Accepted: 27 March 2011 / Published: 4 April 2011

(This article belongs to the Special Issue Lab-on-a-Chip)

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Abstract: We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3–4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.

Keywords: Fluorescence In Situ Hybridization (FISH); lab on chip; genetic analysis; cytogenetics; chromosome spreading; metaphase FISH; chromosomal translocations

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