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Toxins 2017, 9(4), 135; doi:10.3390/toxins9040135

Protein Discovery: Combined Transcriptomic and Proteomic Analyses of Venom from the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae)

1
State Key Laboratory of Rice Biology & Key Laboratory of Agricultural Entomology of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China
2
USDA/Agricultural Research Service Biological Control of Insects Research Laboratory, Columbia, MO 65203, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Michel Dugon
Received: 7 December 2016 / Revised: 28 March 2017 / Accepted: 4 April 2017 / Published: 12 April 2017
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [3921 KB, uploaded 12 April 2017]   |  

Abstract

Many species of endoparasitoid wasps provide biological control services in agroecosystems. Although there is a great deal of information on the ecology and physiology of host/parasitoid interactions, relatively little is known about the protein composition of venom and how specific venom proteins influence physiological systems within host insects. This is a crucial gap in our knowledge because venom proteins act in modulating host physiology in ways that favor parasitoid development. Here, we identified 37 possible venom proteins from the polydnavirus-carrying endoparasitoid Cotesia chilonis by combining transcriptomic and proteomic analyses. The most abundant proteins were hydrolases, such as proteases, peptidases, esterases, glycosyl hydrolase, and endonucleases. Some components are classical parasitoid venom proteins with known functions, including extracellular superoxide dismutase 3, serine protease inhibitor and calreticulin. The venom contains novel proteins, not recorded from any other parasitoid species, including tolloid-like proteins, chitooligosaccharidolytic β-N-acetylglucosaminidase, FK506-binding protein 14, corticotropin-releasing factor-binding protein and vascular endothelial growth factor receptor 2. These new data generate hypotheses and provide a platform for functional analysis of venom components. View Full-Text
Keywords: parasitoid wasps; hosts; venom proteins; venom gland; transcriptomics; proteomics parasitoid wasps; hosts; venom proteins; venom gland; transcriptomics; proteomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Teng, Z.-W.; Xiong, S.-J.; Xu, G.; Gan, S.-Y.; Chen, X.; Stanley, D.; Yan, Z.-C.; Ye, G.-Y.; Fang, Q. Protein Discovery: Combined Transcriptomic and Proteomic Analyses of Venom from the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae). Toxins 2017, 9, 135.

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