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Toxins 2017, 9(4), 137; doi:10.3390/toxins9040137

An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip

1,2,3,4,†
,
1,2,3,4,†,* , 1,2,3
,
1,2,3,4,* , 1,2
,
1,4
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1,2
,
1,3
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1,4
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1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
4
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Massimo Reverberi
Received: 6 February 2017 / Revised: 23 March 2017 / Accepted: 6 April 2017 / Published: 13 April 2017
View Full-Text   |   Download PDF [3118 KB, uploaded 13 April 2017]   |  

Abstract

An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety. View Full-Text
Keywords: total aflatoxin; quantum dot nanobead; test strip; on-site; peanut and rice; agro-food safety total aflatoxin; quantum dot nanobead; test strip; on-site; peanut and rice; agro-food safety
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Ouyang, S.; Zhang, Z.; He, T.; Li, P.; Zhang, Q.; Chen, X.; Wang, D.; Li, H.; Tang, X.; Zhang, W. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins 2017, 9, 137.

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