Next Article in Journal
Monitoring the Temporal Expression of Genes Involved in Ochratoxin A Production of Aspergillus carbonarius under the Influence of Temperature and Water Activity
Next Article in Special Issue
Chronic Exposure to the Fusarium Mycotoxin Deoxynivalenol: Impact on Performance, Immune Organ, and Intestinal Integrity of Slow-Growing Chickens
Previous Article in Journal
Understanding the Mechanism of Translocation of Adenylate Cyclase Toxin across Biological Membranes
Previous Article in Special Issue
Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Toxins 2017, 9(10), 294; doi:10.3390/toxins9100294

Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin

1
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
2
Laboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan
3
Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan
4
Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan
5
Department of Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka
6
MILS International, Kanazawa 920-0222, Japan
7
Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan
8
Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, The Netherlands
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 1 May 2017 / Revised: 14 September 2017 / Accepted: 18 September 2017 / Published: 21 September 2017
(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)
View Full-Text   |   Download PDF [557 KB, uploaded 21 September 2017]   |  

Abstract

The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals (n = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550–600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550–600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle. View Full-Text
Keywords: cattle; GC/MS; metabolomics; sterigmatocystin; urine; zearalenone cattle; GC/MS; metabolomics; sterigmatocystin; urine; zearalenone
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Toda, K.; Kokushi, E.; Uno, S.; Shiiba, A.; Hasunuma, H.; Fushimi, Y.; Wijayagunawardane, M.P.B.; Zhang, C.; Yamato, O.; Taniguchi, M.; Fink-Gremmels, J.; Takagi, M. Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin. Toxins 2017, 9, 294.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top