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Toxins 2017, 9(1), 5; doi:10.3390/toxins9010005

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

1
Physiology Department, University Federal of Rio Grande of Norte, Natal, RN 59078-970, Brazil
2
Biology Department, University of São Paulo, Ribeirao Preto, SP 14040-901, Brazil
3
Biophysics Department, Federal University of São Paulo, São Paulo, SP 04023-062, Brazil
4
Pharmacology Department, Federal University of São Paulo, São Paulo, SP 04023-062, Brazil
5
Biosciences Department, Federal University of São Paulo, Santos, SP 11015-020, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Eivind Undheim
Received: 23 May 2016 / Revised: 24 October 2016 / Accepted: 1 November 2016 / Published: 23 December 2016
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [2232 KB, uploaded 23 December 2016]   |  

Abstract

Natural products, sources of new pharmacological substances, have large chemical diversity and architectural complexity. In this context, some toxins obtained from invertebrate venoms have anticonvulsant effects. Epilepsy is a neurological disorder that affects about 65 million people worldwide, and approximately 30% of cases are resistant to pharmacological treatment. Previous studies from our group show that the denatured venom of the ant Dinoponera quadriceps (Kempt) protects mice against bicuculline (BIC)-induced seizures and death. The aim of this study was to investigate the anticonvulsant activity of compounds isolated from D. quadriceps venom against seizures induced by BIC in mice. Crude venom was fractionated by high-performance liquid chromatography (HPLC) resulting in six fractions referred to as DqTx1–DqTx6. A liquid chromatography-mass spectrometry (LC/MS) analysis revealed a major 431 Da compound in fractions DqTx1 and DqTx2. Fractions DqTx3 and DqTx4 showed a compound of 2451 Da and DqTx5 revealed a 2436 Da compound. Furthermore, the DqTx6 fraction exhibited a major component with a molecular weight of 13,196 Da. Each fraction (1 mg/mL) was microinjected into the lateral ventricle of mice, and the animals were observed in an open field. We did not observe behavioral alterations when the fractions were given alone. Conversely, when the fractions were microinjected 20 min prior to the administration of BIC (21.6 nM), DqTx1, DqTx4, and DqTx6 fractions increased the latency for onset of tonic-clonic seizures. Moreover, all fractions, except DqTx5, increased latency to death. The more relevant result was obtained with the DqTx6 fraction, which protected 62.5% of the animals against tonic-clonic seizures. Furthermore, this fraction protected 100% of the animals from seizure episodes followed by death. Taken together, these findings indicate that compounds from ant venom might be a potential source of new anticonvulsants molecules. View Full-Text
Keywords: ant venom; neuroactive compounds; bicuculline; tonic-clonic seizures; peptide fraction; natural product ant venom; neuroactive compounds; bicuculline; tonic-clonic seizures; peptide fraction; natural product
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nôga, D.A.M.F.; Brandão, L.E.M.; Cagni, F.C.; Silva, D.; de Azevedo, D.L.O.; Araújo, A.; dos Santos, W.F.; Miranda, A.; da Silva, R.H.; Ribeiro, A.M. Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae). Toxins 2017, 9, 5.

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