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Toxins 2016, 8(4), 105; doi:10.3390/toxins8040105

Quantitative Proteomic Analysis of Venoms from Russian Vipers of Pelias Group: Phospholipases A2 are the Main Venom Components

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia
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Academic Editor: Stephen P. Mackessy
Received: 15 February 2016 / Revised: 28 March 2016 / Accepted: 5 April 2016 / Published: 12 April 2016
(This article belongs to the Special Issue Venomics, Venom Proteomics and Venom Transcriptomics)
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Abstract

Venoms of most Russian viper species are poorly characterized. Here, by quantitative chromato-mass-spectrometry, we analyzed protein and peptide compositions of venoms from four Vipera species (V. kaznakovi, V. renardi, V. orlovi and V. nikolskii) inhabiting different regions of Russia. In all these species, the main components were phospholipases A2, their content ranging from 24% in V. orlovi to 65% in V. nikolskii. Altogether, enzyme content in venom of V. nikolskii reached ~85%. Among the non-enzymatic proteins, the most abundant were disintegrins (14%) in the V. renardi venom, C-type lectin like (12.5%) in V. kaznakovi, cysteine-rich venom proteins (12%) in V. orlovi and venom endothelial growth factors (8%) in V. nikolskii. In total, 210 proteins and 512 endogenous peptides were identified in the four viper venoms. They represented 14 snake venom protein families, most of which were found in the venoms of Vipera snakes previously. However, phospholipase B and nucleotide degrading enzymes were reported here for the first time. Compositions of V. kaznakovi and V. orlovi venoms were described for the first time and showed the greatest similarity among the four venoms studied, which probably reflected close relationship between these species within the “kaznakovi” complex. View Full-Text
Keywords: snake venom; viper; Vipera kaznakovi; Vipera nikolskii; Vipera orlovi; Vipera renardi; proteome; mass-spectrometry snake venom; viper; Vipera kaznakovi; Vipera nikolskii; Vipera orlovi; Vipera renardi; proteome; mass-spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kovalchuk, S.I.; Ziganshin, R.H.; Starkov, V.G.; Tsetlin, V.I.; Utkin, Y.N. Quantitative Proteomic Analysis of Venoms from Russian Vipers of Pelias Group: Phospholipases A2 are the Main Venom Components. Toxins 2016, 8, 105.

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