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Toxins 2016, 8(11), 336; doi:10.3390/toxins8110336

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

1
Chemistry Department, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada
2
Institute of Sciences of Food Production, National Research Council of Italy, via G. Amendola 122/O, Bari 70126, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 5 October 2016 / Revised: 2 November 2016 / Accepted: 8 November 2016 / Published: 15 November 2016
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
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Abstract

Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications. View Full-Text
Keywords: aptamer; ochratoxin A; mycotoxins; biorecognition; fluorescent assay; biosensing; aptasensor; microscale thermophoresis aptamer; ochratoxin A; mycotoxins; biorecognition; fluorescent assay; biosensing; aptasensor; microscale thermophoresis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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McKeague, M.; Velu, R.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A. Toxins 2016, 8, 336.

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