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Comment published on 14 February 2017, see Toxins 2017, 9(2), 65.

Open AccessArticle
Toxins 2016, 8(11), 333; doi:10.3390/toxins8110333

Screening and Identification of Novel Ochratoxin A-Producing Fungi from Grapes

1
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China
2
Hubei Key Laboratory, Edible Wild Plants Conservation and Utilization, 11 Cihu Road, Huangshi 435002, China
3
School of Grain Science and Technology, Jiangsu University of Science and Technology, 2 Mengxi Road, Zhenjiang 212003, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Richard A. Manderville
Received: 27 September 2016 / Revised: 24 October 2016 / Accepted: 8 November 2016 / Published: 12 November 2016
(This article belongs to the Collection Ochratoxins-Collection)
View Full-Text   |   Download PDF [1827 KB, uploaded 12 November 2016]   |  

Abstract

Ochratoxin A (OTA) contamination has been established as a world-wide problem. In this study, the strains with the ability of OTA production were screened by analyzing the green fluorescence of the isolates colonies from the grapes in Zhenjiang with 365 nm UV light and confirmed by HPLC with fluorescent detection (HPLC-FLD). The results showed that seven isolates acquired the characteristic of the fluorescence, of which only five showed the ability of OTA production as confirmed by HPLC-FLD analysis. The five OTA-producing strains were identified based on comparative sequence analysis of three conserved genes (ITS, BenA and RPB2) of the strains, and they are Talaromyces rugulosus (O1 and Q3), Penicillium commune (V5-1), Penicillium rubens (MQ-5) and Aspergillus aculeatus (MB1-1). There are two Penicillium species of the five OTA-producing strains and our study is the first to report that P. rubens, T. rugulosus and A. aculeatus can produce OTA. This work would contribute to comprehensively understanding the fungi with an OTA-producing ability in grapes before harvest and then take effective measures to prevent OTA production. View Full-Text
Keywords: screening; identification; ochratoxin A (OTA); fungi; grapes screening; identification; ochratoxin A (OTA); fungi; grapes
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Zhang, X.; Li, Y.; Wang, H.; Gu, X.; Zheng, X.; Wang, Y.; Diao, J.; Peng, Y.; Zhang, H. Screening and Identification of Novel Ochratoxin A-Producing Fungi from Grapes. Toxins 2016, 8, 333.

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