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Toxins 2015, 7(2), 604-620; doi:10.3390/toxins7020604

Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight

1
Kihara Institute for Biological Research, Yokohama City University, Maioka 641-12, Totsuka, Yokohama 244-0813, Japan
2
Vascular Biology Laboratory, Heart Institute (InCor), University of Sao Paulo, School of Medicine, Av. Eneas C Aguiar, 44-Annex 2, 9th floor, Sao Paulo 05403-900, Brazil
3
National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba 305-8642, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Antonio Moretti
Received: 30 December 2014 / Revised: 3 February 2015 / Accepted: 10 February 2015 / Published: 16 February 2015
(This article belongs to the Section Mycotoxins)
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Abstract

Fusarium graminearum is responsible for Fusarium head blight (FHB), which is a destructive disease of wheat that makes its quality unsuitable for end use. To understand the temporal molecular response against this pathogen, microarray gene expression analysis was carried out at two time points on three wheat genotypes, the spikes of which were infected by Fusarium graminearum. The greatest number of genes was upregulated in Nobeokabouzu-komugi followed by Sumai 3, whereas the minimum expression in Gamenya was at three days after inoculation (dai). In Nobeokabouzu-komugi, high expression of detoxification genes, such as multidrug-resistant protein, multidrug resistance-associated protein, UDP-glycosyltransferase and ABC transporters, in addition to systemic defense-related genes, were identified at the early stage of infection. This early response of the highly-resistant genotype implies a different resistance response from the other resistant genotype, Sumai 3, primarily containing local defense-related genes, such as cell wall defense genes. In Gamenya, the expression of all three functional groups was minimal. The differences in these molecular responses with respect to the time points confirmed the variation in the genotypes. For the first time, we report the nature of gene expression in the FHB-highly resistant cv. Nobeokabouzu-komugi during the disease establishment stage and the possible underlying molecular response. View Full-Text
Keywords: wheat; Fusarium graminearum; microarray; molecular response; detoxification; local and systemic response wheat; Fusarium graminearum; microarray; molecular response; detoxification; local and systemic response
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kosaka, A.; Manickavelu, A.; Kajihara, D.; Nakagawa, H.; Ban, T. Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight. Toxins 2015, 7, 604-620.

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