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Toxins 2015, 7(11), 4455-4467; doi:10.3390/toxins7114455

The Saccharomyces boulardii CNCM I-745 Strain Shows Protective Effects against the B. anthracis LT Toxin

Centre Scientifique de Monaco, Monaco 98000, Monaco
Team Inflammation, Cancer, Cancer Stem Cells, Centre Méditerranéen de Médecine Moléculaire (C3M), INSERM, U1065, Nice 06204, France
Faculté de Médecine, UFR Médecine, IFR50, Université de Nice-Sophia Antipolis, UNSA, Nice 06204, France
Team Microbial Toxins in Host Pathogen Interactions, Centre Méditerranéen de Médecine Moléculaire (C3M), INSERM, U1065, Nice 06204, France
Author to whom correspondence should be addressed.
Academic Editor: Shihui Liu
Received: 22 July 2015 / Revised: 15 October 2015 / Accepted: 26 October 2015 / Published: 30 October 2015
(This article belongs to the Collection Anthrax Toxins)
View Full-Text   |   Download PDF [3837 KB, uploaded 30 October 2015]   |  


The probiotic yeast Saccharomyces boulardii (S. boulardii) has been prescribed for the prophylaxis and treatment of several infectious diarrheal diseases. Gastrointestinal anthrax causes fatal systemic disease. In the present study, we investigated the protective effects conferred by Saccharomyces boulardii CNCM I-745 strain on polarized T84 columnar epithelial cells intoxicated by the lethal toxin (LT) of Bacillus anthracis. Exposure of polarized T84 cells to LT affected cell monolayer integrity, modified the morphology of tight junctions and induced the formation of actin stress fibers. Overnight treatment of cells with S. boulardii before incubation with LT maintained the integrity of the monolayers, prevented morphological modification of tight junctions, restricted the effects of LT on actin remodeling and delayed LT-induced MEK-2 cleavage. Mechanistically, we demonstrated that in the presence of S. boulardii, the medium is depleted of both LF and PA sub-units of LT and the appearance of a cleaved form of PA. Our study highlights the potential of the S. boulardii CNCM I-745 strain as a prophylactic agent against the gastrointestinal form of anthrax. View Full-Text
Keywords: anthrax toxins; probiotics; Saccharomyces boulardii anthrax toxins; probiotics; Saccharomyces boulardii

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pontier-Bres, R.; Rampal, P.; Peyron, J.-F.; Munro, P.; Lemichez, E.; Czerucka, D. The Saccharomyces boulardii CNCM I-745 Strain Shows Protective Effects against the B. anthracis LT Toxin. Toxins 2015, 7, 4455-4467.

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