Toxins 2014, 6(4), 1169-1176; doi:10.3390/toxins6041169
Article

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Received: 20 January 2014; in revised form: 28 February 2014 / Accepted: 11 March 2014 / Published: 26 March 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Acetogenins are secondary metabolites exclusively produced by Annonaceae, which have antitumor, cytotoxic, and pesticide activities. In this study, we evaluated the larvicidal and cytotoxic effect of squamocin from Annona squamosa on Aedes aegypti (Diptera: Culicidae) midgut. The compound was solubilized in 2% Tween 20 at 10, 20, 50, 80 and 100 ppm. The assay was conducted in a completely randomized design with four replications, each with 20 third-instar larvae. Larval mortality was assessed every hour until total mortality, and the data were subjected to Probit analysis. Cellular damage was evaluated every 30 min in groups comprising five larvae subjected to squamocin at 50 and 100 ppm for 240 min. The total larval mortality occurred after 360 min following application of 50, 80, and 100 ppm squamocin, and 600 min after applying other concentrations with LC50 at 6.4 ppm. Both 50 and 100 ppm of squamocin showed cytotoxic activity in the midgut epithelium of A. aegypti after 240 min with 50 ppm resulting in midgut cells with light cytoplasm containing small vacuoles, whereas at 100 ppm were found cells with cytoplasm highly vacuolated, damaged apical surface and cell protrusion toward the gut lumen. In conclusion, squamocin has the potential to control A. aegypti.
Keywords: acetogenin; Annonaceae; cell death; botanical insecticides; dengue
PDF Full-text Download PDF Full-Text [2963 KB, uploaded 26 March 2014 11:30 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Costa, M.S.; Cossolin, J.F.S.; Pereira, M.J.B.; Sant'Ana, A.E.G.; Lima, M.D.; Zanuncio, J.C.; Serrão, J.E. Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae). Toxins 2014, 6, 1169-1176.

AMA Style

Costa MS, Cossolin JFS, Pereira MJB, Sant'Ana AEG, Lima MD, Zanuncio JC, Serrão JE. Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae). Toxins. 2014; 6(4):1169-1176.

Chicago/Turabian Style

Costa, Marilza S.; Cossolin, Jamile F.S.; Pereira, Mônica J.B.; Sant'Ana, Antônio E.G.; Lima, Milena D.; Zanuncio, José C.; Serrão, José E. 2014. "Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)." Toxins 6, no. 4: 1169-1176.

Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert