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Toxins 2013, 5(9), 1587-1596; doi:10.3390/toxins5091587

Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin

1 Center for Coastal Studies Texas A&M University Corpus Christi 6300 Ocean Drive Corpus Christi, TX 78412, USA 2 Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA 3 NOAA/NCCOS Center for Human Health Research Hollings Marine Laboratory, 331 Fort Johnson Rd, Charleston, SC 29412, USA 4 Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
* Author to whom correspondence should be addressed.
Received: 24 July 2013 / Revised: 5 September 2013 / Accepted: 6 September 2013 / Published: 18 September 2013
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Euglenophycin is a recently discovered toxin produced by at least one species of euglenoid algae. The toxin has been responsible for several fish mortality events. To facilitate the identification and monitoring of euglenophycin in freshwater ponds, we have developed a specific mass spectrometric method for the identification and quantitation of euglenophycin. The post-extraction stability of the toxin was assessed under various conditions. Euglenophycin was most stable at room temperature. At 8 °C there was a small, but statistically significant, loss in toxin after one day. These methods and knowledge of the toxin’s stability will facilitate identification of the toxin as a causative agent in fish kills and determination of the toxin’s distribution in the organs of exposed fish.
Keywords: euglena; euglenophycin; stability; toxin euglena; euglenophycin; stability; toxin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gutierrez, D.B.; Rafalski, A.; Beauchesne, K.; Moeller, P.D.; Triemer, R.E.; Zimba, P.V. Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin. Toxins 2013, 5, 1587-1596.

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