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Toxins 2013, 5(2), 327-335; doi:10.3390/toxins5020327
Communication

ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

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Received: 3 September 2012; in revised form: 13 December 2012 / Accepted: 16 December 2012 / Published: 18 February 2013
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Abstract: A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.
Keywords: bioactive peptide; BPP; pyroglutamic acid; pyrrolidonecarboxylic acid; de novo peptide sequencing bioactive peptide; BPP; pyroglutamic acid; pyrrolidonecarboxylic acid; de novo peptide sequencing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Coutinho-Neto, A.; Caldeira, C.A.S.; Souza, G.H.M.F.; Zaqueo, K.D.; Kayano, A.M.; Silva, R.S.; Zuliani, J.P.; Soares, A.M.; Stábeli, R.G.; Calderon, L.A. ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer. Toxins 2013, 5, 327-335.

AMA Style

Coutinho-Neto A, Caldeira CAS, Souza GHMF, Zaqueo KD, Kayano AM, Silva RS, Zuliani JP, Soares AM, Stábeli RG, Calderon LA. ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer. Toxins. 2013; 5(2):327-335.

Chicago/Turabian Style

Coutinho-Neto, Antonio; Caldeira, Cleópatra A.S.; Souza, Gustavo H.M.F.; Zaqueo, Kayena D.; Kayano, Anderson M.; Silva, Rodrigo S.; Zuliani, Juliana P.; Soares, Andreimar M.; Stábeli, Rodrigo G.; Calderon, Leonardo A. 2013. "ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer." Toxins 5, no. 2: 327-335.


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