Toxins 2013, 5(2), 327-335; doi:10.3390/toxins5020327
Communication

ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

Antonio Coutinho-Neto 1 , Cleópatra A. S. Caldeira 1 email, Gustavo H. M. F. Souza 2 email, Kayena D. Zaqueo 1 email, Anderson M. Kayano 1 email, Rodrigo S. Silva 1 email, Juliana P. Zuliani 1,3 email, Andreimar M. Soares 1 email, Rodrigo G. Stábeli 1,3 email and Leonardo A. Calderon 1,3,* email
1 Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Porto Velho, RO 76820-245, Brazil 2 MS Applications Development Laboratory, Waters Corporation, Alphaville, SP 06455-020, Brazil 3 Medicine Department, Federal University of Rondônia, Porto Velho, RO 76801-059, Brazil
* Author to whom correspondence should be addressed.
Received: 3 September 2012; in revised form: 13 December 2012 / Accepted: 16 December 2012 / Published: 18 February 2013
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Abstract: A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.
Keywords: bioactive peptide; BPP; pyroglutamic acid; pyrrolidonecarboxylic acid; de novo peptide sequencing

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Cite This Article

MDPI and ACS Style

Coutinho-Neto, A.; Caldeira, C.A.S.; Souza, G.H.M.F.; Zaqueo, K.D.; Kayano, A.M.; Silva, R.S.; Zuliani, J.P.; Soares, A.M.; Stábeli, R.G.; Calderon, L.A. ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer. Toxins 2013, 5, 327-335.

AMA Style

Coutinho-Neto A, Caldeira CAS, Souza GHMF, Zaqueo KD, Kayano AM, Silva RS, Zuliani JP, Soares AM, Stábeli RG, Calderon LA. ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer. Toxins. 2013; 5(2):327-335.

Chicago/Turabian Style

Coutinho-Neto, Antonio; Caldeira, Cleópatra A.S.; Souza, Gustavo H.M.F.; Zaqueo, Kayena D.; Kayano, Anderson M.; Silva, Rodrigo S.; Zuliani, Juliana P.; Soares, Andreimar M.; Stábeli, Rodrigo G.; Calderon, Leonardo A. 2013. "ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer." Toxins 5, no. 2: 327-335.

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