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Toxins 2013, 5(2), 203-223; doi:10.3390/toxins5020203
Article

Interactions of PLA2-s from Vipera lebetina, Vipera berus berus and Naja naja oxiana Venom with Platelets, Bacterial and Cancer Cells

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Received: 7 December 2012; in revised form: 16 January 2013 / Accepted: 17 January 2013 / Published: 24 January 2013
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Abstract: Secretory phospholipasesA2 (sPLA2s) form a large family of structurally related enzymes widespread in nature. Herein, we studied the inhibitory effects of sPLA2s from Vipera lebetina (VLPLA2), Vipera berus berus (VBBPLA2), and Naja naja oxiana (NNOPLA2) venoms on (i) human platelets, (ii) four different bacterial strains (gram-negative Escherichia coli and Vibrio fischeri; gram-positive Staphylococcus aureus and Bacillus subtilis) and (iii) five types of cancer cells (PC-3, LNCaP, MCF-7, K-562 and B16-F10) in vitro. sPLA2s inhibited collagen-induced platelet aggregation: VBBPLA2 IC50 = 0.054, VLPLA2 IC50 = 0.072, NNOPLA2 IC50 = 0.814 μM. p-Bromophenacylbromide-inhibited sPLA2 had no inhibitory action on platelets. 36.17 μM VBBPLA2 completely inhibited the growth of gram-positive Bacillus subtilis whereas no growth inhibition was observed towards gram-negative Escherichia coli. The inhibitory action of sPLA2s (~0.7 μM and ~7 μM) towards cancer cells depended on both venom and cell type. VBBPLA2 (7.2 μM) inhibited significantly the viability of K-562 cells and the cell death appeared apoptotic. The sPLA2s exhibited no inhibitory effect towards LNCaP cells and some effect (8%–20%) towards other cells. Thus, already sub-μM concentrations of sPLA2s inhibited collagen-induced platelet aggregation and from the current suite of studied svPLA2s and test cells, VBBPLA2 was the most growth inhibitory towards Bacillus subtilis and K-562 cells.
Keywords: snake venom; Vipera lebetina; Vipera berus berus; Naja naja oxiana; phospholipase A2; human platelet aggregation inhibition; antibacterial; bioluminescent bacteria; cancer cells snake venom; Vipera lebetina; Vipera berus berus; Naja naja oxiana; phospholipase A2; human platelet aggregation inhibition; antibacterial; bioluminescent bacteria; cancer cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Samel, M.; Vija, H.; Kurvet, I.; Künnis-Beres, K.; Trummal, K.; Subbi, J.; Kahru, A.; Siigur, J. Interactions of PLA2-s from Vipera lebetina, Vipera berus berus and Naja naja oxiana Venom with Platelets, Bacterial and Cancer Cells. Toxins 2013, 5, 203-223.

AMA Style

Samel M, Vija H, Kurvet I, Künnis-Beres K, Trummal K, Subbi J, Kahru A, Siigur J. Interactions of PLA2-s from Vipera lebetina, Vipera berus berus and Naja naja oxiana Venom with Platelets, Bacterial and Cancer Cells. Toxins. 2013; 5(2):203-223.

Chicago/Turabian Style

Samel, Mari; Vija, Heiki; Kurvet, Imbi; Künnis-Beres, Kai; Trummal, Katrin; Subbi, Juhan; Kahru, Anne; Siigur, Jüri. 2013. "Interactions of PLA2-s from Vipera lebetina, Vipera berus berus and Naja naja oxiana Venom with Platelets, Bacterial and Cancer Cells." Toxins 5, no. 2: 203-223.



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