Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Toxins 2011, 3(8), 979-990; doi:10.3390/toxins3080979
Article

Mechanism of Lethal Toxin Neutralization by a Human Monoclonal Antibody Specific for the PA20 Region of Bacillus anthracis Protective Antigen

* ,
,
,
 and
Received: 29 April 2011; in revised form: 7 July 2011 / Accepted: 4 August 2011 / Published: 9 August 2011
(This article belongs to the Special Issue Anthrax Toxin)
View Full-Text   |   Download PDF [302 KB, uploaded 9 August 2011]
Abstract: The primary immunogenic component of the currently approved anthrax vaccine is the protective antigen (PA) unit of the binary toxin system. PA-specific antibodies neutralize anthrax toxins and protect against infection. Recent research has determined that in humans, only antibodies specific for particular determinants are capable of effecting toxin neutralization, and that the neutralizing epitopes recognized by these antibodies are distributed throughout the PA monomer. The mechanisms by which the majority of these epitopes effect neutralization remain unknown. In this report we investigate the process by which a human monoclonal antibody specific for the amino-terminal domain of PA neutralizes lethal toxin in an in vitro assay of cytotoxicity, and find that it neutralizes LT by blocking the requisite cleavage of the amino-terminal 20 kD portion of the molecule (PA20) from the remainder of the PA monomer. We also demonstrate that the epitope recognized by this human monoclonal does not encompass the 166RKKR169 furin recognition sequence in domain 1 of PA.
Keywords: anthrax; Bacillus anthracis; antibody epitope; paratope; human monoclonal antibody; protective antigen; furin; toxin neutralization anthrax; Bacillus anthracis; antibody epitope; paratope; human monoclonal antibody; protective antigen; furin; toxin neutralization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Reason, D.; Liberato, J.; Sun, J.; Camacho, J.; Zhou, J. Mechanism of Lethal Toxin Neutralization by a Human Monoclonal Antibody Specific for the PA20 Region of Bacillus anthracis Protective Antigen. Toxins 2011, 3, 979-990.

AMA Style

Reason D, Liberato J, Sun J, Camacho J, Zhou J. Mechanism of Lethal Toxin Neutralization by a Human Monoclonal Antibody Specific for the PA20 Region of Bacillus anthracis Protective Antigen. Toxins. 2011; 3(8):979-990.

Chicago/Turabian Style

Reason, Donald; Liberato, Justine; Sun, Jinying; Camacho, Jessica; Zhou, Jianhui. 2011. "Mechanism of Lethal Toxin Neutralization by a Human Monoclonal Antibody Specific for the PA20 Region of Bacillus anthracis Protective Antigen." Toxins 3, no. 8: 979-990.



Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert