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Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON)
Institute of Food Safety, Jiangsu Academy of Agriculture Science (Key Lab of Agro-Food Safety and Quality, Ministry of Agriculture; Key Lab of Food Quality and Safety of Jiangsu Province—State Key Laboratory Breeding Base), Nanjing 210014, China
* Author to whom correspondence should be addressed.
Received: 8 May 2011; in revised form: 28 June 2011 / Accepted: 14 July 2011 / Published: 4 August 2011
Abstract: Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high risk of DON ingestion and the possibility of frequent exposure, it is important to develop a rapid and highly sensitive method for easy identification and quantification of DON in grain samples. In this study, we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) to detect DON in wheat. We conjugated 3-O-Hemisuccinyl-DON (3HS-DON) to Bovine serum albumin (BSA) and Ovalbumin (OVA), and obtained DON-specific mice antisera. The indirect competitive ELISA revealed that the optimal concentration of mice serum and the coated antigen was 1/1600 and 1/1500, respectively. The antiserum cross-reacted with the trichothecenes 3-acetyl-DON and T-2 toxin, reaching about 55.2% and 6.3%, respectively, as compared with DON. Results showed that the assay could be performed satisfactorily using an extraction buffer containing less than 15% methanol. Recovery from DON was 82–93% in grains. The linear detection range of DON in grains was between 0.01 and 100 μg/mL.
Keywords: Deoxynivalenol (DON); immunoassay; ELISA; Fusarium graminearum
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MDPI and ACS Style
Ji, F.; Li, H.; Xu, J.; Shi, J. Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON). Toxins 2011, 3, 968-978.
Ji F, Li H, Xu J, Shi J. Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON). Toxins. 2011; 3(8):968-978.
Ji, Fang; Li, Hua; Xu, Jianhong; Shi, Jianrong. 2011. "Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON)." Toxins 3, no. 8: 968-978.