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Expression Analysis of Stress-Related Genes in Kernels of Different Maize (Zea mays L.) Inbred Lines with Different Resistance to Aflatoxin Contamination
Department of Plant Pathology, The University of Georgia, Tifton, GA 31793, USA
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China
Department of Crop and Soil Sciences, The University of Georgia, Tifton, GA 31793, USA
Department of Plant Pathology and Crop Physiology, Louisiana State University, Baton Rouge, LA 70803, USA
United States Department of Agriculture-Agriculture Research Service, Biological Control of Pests Research Unit, Stoneville, MS 38776, USA
United States Department of Agriculture-Agriculture Research Service, Crop Protection and Management Research Unit, Tifton, GA 31793, USA
* Author to whom correspondence should be addressed.
Received: 27 April 2011; in revised form: 10 May 2011 / Accepted: 14 May 2011 / Published: 9 June 2011
(This article belongs to the Special Issue Aflatoxins
Abstract: This research examined the expression patterns of 94 stress-related genes in seven maize inbred lines with differential expressions of resistance to aflatoxin contamination. The objective was to develop a set of genes/probes associated with resistance to A. flavus and/or aflatoxin contamination. Ninety four genes were selected from previous gene expression studies with abiotic stress to test the differential expression in maize lines, A638, B73, Lo964, Lo1016, Mo17, Mp313E, and Tex6, using real-time RT-PCR. Based on the relative-expression levels, the seven maize inbred lines clustered into two different groups. One group included B73, Lo1016 and Mo17, which had higher levels of aflatoxin contamination and lower levels of overall gene expression. The second group which included Tex6, Mp313E, Lo964 and A638 had lower levels of aflatoxin contamination and higher overall levels of gene expressions. A total of six “cross-talking” genes were identified between the two groups, which are highly expressed in the resistant Group 2 but down-regulated in susceptible Group 1. When further subjected to drought stress, Tex6 expressed more genes up-regulated and B73 has fewer genes up-regulated. The transcript patterns and interactions measured in these experiments indicate that the resistant mechanism is an interconnected process involving many gene products and transcriptional regulators, as well as various host interactions with environmental factors, particularly, drought and high temperature.
Keywords: resistance genes; gene expression; qPCR; aflatoxin contamination
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Jiang, T.; Zhou, B.; Luo, M.; Abbas, H.K.; Kemerait, R.; Lee, R.D.; Scully, B.T.; Guo, B. Expression Analysis of Stress-Related Genes in Kernels of Different Maize (Zea mays L.) Inbred Lines with Different Resistance to Aflatoxin Contamination. Toxins 2011, 3, 538-550.
Jiang T, Zhou B, Luo M, Abbas HK, Kemerait R, Lee RD, Scully BT, Guo B. Expression Analysis of Stress-Related Genes in Kernels of Different Maize (Zea mays L.) Inbred Lines with Different Resistance to Aflatoxin Contamination. Toxins. 2011; 3(6):538-550.
Jiang, Tingbo; Zhou, Boru; Luo, Meng; Abbas, Hamed K.; Kemerait, Robert; Lee, Robert Dewey; Scully, Brian T.; Guo, Baozhu. 2011. "Expression Analysis of Stress-Related Genes in Kernels of Different Maize (Zea mays L.) Inbred Lines with Different Resistance to Aflatoxin Contamination." Toxins 3, no. 6: 538-550.