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• Bazin, I
• Nabais, E
• Lopez-Ferber, M
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• Nabais, E
• Lopez-Ferber, M
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• Nabais, E
• Lopez-Ferber, M

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Toxins 2010, 2(9), 2230-2241; doi:10.3390/toxins2092230

Review

Rapid Visual Tests: Fast and Reliable Detection of Ochratoxin A

Ingrid Bazin* , Elodie Nabais and Miguel Lopez-Ferber

Ecole des Mines d’Ales, 6 av. de Clavieres 30319, Ales cedex, France

* Author to whom correspondence should be addressed.

Received: 2 August 2010; in revised form: 13 August 2010 / Accepted: 23 August 2010 / Published: 26 August 2010

(This article belongs to the Special Issue Ochratoxins)

Download PDF Full-Text [394 KB, uploaded 26 August 2010 14:58 CEST]

Abstract: This paper reviews the early detection strategies that have been employed for the rapid monitoring of ochratoxin A (OTA) contamination of food. OTA, a mycotoxin mainly produced by some Aspergillus and Penicillium species, is found in cereals, coffee, wine, pork and grapes. To minimize the entry of this mycotoxin into the food chain, rapid diagnostic tools are required. To this end, the potential use of lateral flow devices has also been developed. In this study, we analyze the robustness of test strips using published methods for colorimetric detection. Different test formats are discussed, and challenges in the development of lateral flow devices for on-site determination of OTA, with requirements such as robustness, speed, and cost-effectiveness, are discussed.

Keywords: ochratoxin A; colorimetric test; lateral flow test; flow-through test; clean-up immunoassay

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