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Toxins 2010, 2(6), 1582-1594; doi:10.3390/toxins2061582

Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany
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Received: 26 March 2010 / Revised: 8 June 2010 / Accepted: 18 June 2010 / Published: 21 June 2010
(This article belongs to the Special Issue Ochratoxins)
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Abstract

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
Keywords: mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis
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MDPI and ACS Style

Heussner, A.H.; Ausländer, S.; Dietrich, D.R. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. Toxins 2010, 2, 1582-1594.

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