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• Ausländer, S
• Dietrich, D
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Toxins 2010, 2(6), 1582-1594; doi:10.3390/toxins2061582

Article

Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Alexandra H. Heussner, Simon Ausländer and Daniel R. Dietrich*

Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany

* Author to whom correspondence should be addressed.

Received: 26 March 2010; in revised form: 8 June 2010 / Accepted: 18 June 2010 / Published: 21 June 2010

(This article belongs to the Special Issue Ochratoxins)

Download PDF Full-Text [239 KB, uploaded 21 June 2010 13:51 CEST]

Abstract: A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

Keywords: mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis

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