Next Article in Journal
Specificity of Interaction between Clostridium perfringens Enterotoxin and Claudin-Family Tight Junction Proteins
Next Article in Special Issue
Chemical, Physical and Biological Approaches to Prevent Ochratoxin Induced Toxicoses in Humans and Animals
Previous Article in Journal
Arsenic in Cancer Treatment: Challenges for Application of Realgar Nanoparticles (A Minireview)
Previous Article in Special Issue
Molecularly Imprinted Polymers for Ochratoxin A Extraction and Analysis
Toxins 2010, 2(6), 1582-1594; doi:10.3390/toxins2061582
Article

Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

,
 and
*
Received: 26 March 2010 / Revised: 8 June 2010 / Accepted: 18 June 2010 / Published: 21 June 2010
(This article belongs to the Special Issue Ochratoxins)
Download PDF [239 KB, uploaded 21 June 2010]

Abstract

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
Keywords: mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Heussner, A.H.; Ausländer, S.; Dietrich, D.R. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. Toxins 2010, 2, 1582-1594.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert