Toxins 2010, 2(6), 1582-1594; doi:10.3390/toxins2061582
Article

Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany
* Author to whom correspondence should be addressed.
Received: 26 March 2010; in revised form: 8 June 2010 / Accepted: 18 June 2010 / Published: 21 June 2010
(This article belongs to the Special Issue Ochratoxins)
PDF Full-text Download PDF Full-Text [239 KB, uploaded 21 June 2010 13:51 CEST]
Abstract: A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
Keywords: mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Heussner, A.H.; Ausländer, S.; Dietrich, D.R. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. Toxins 2010, 2, 1582-1594.

AMA Style

Heussner AH, Ausländer S, Dietrich DR. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. Toxins. 2010; 2(6):1582-1594.

Chicago/Turabian Style

Heussner, Alexandra H.; Ausländer, Simon; Dietrich, Daniel R. 2010. "Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA." Toxins 2, no. 6: 1582-1594.

Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert