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Toxins 2010, 2(6), 1265-1278; https://doi.org/10.3390/toxins2061265

Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts

1
Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Via Trentacoste 2, I-20134 Milan, Italy
2
Università Telematica San Raffaele, Roma, Via di Val Cannuta, 247, 00166 Roma, Italy
3
Department of Animal Health and Bioscience University of Aarhus, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
*
Author to whom correspondence should be addressed.
Received: 22 April 2010 / Revised: 14 May 2010 / Accepted: 31 May 2010 / Published: 1 June 2010
(This article belongs to the Special Issue Ochratoxins)
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Abstract

The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane. View Full-Text
Keywords: ochratoxin A; α-tocopherol; DNA damage; fibroblasts; swine ochratoxin A; α-tocopherol; DNA damage; fibroblasts; swine
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Fusi, E.; Rebucci, R.; Pecorini, C.; Campagnoli, A.; Pinotti, L.; Saccone, F.; Cheli, F.; Purup, S.; Sejrsen, K.; Baldi, A. Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts. Toxins 2010, 2, 1265-1278.

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