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Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Via Trentacoste 2, I-20134 Milan, Italy
Università Telematica San Raffaele, Roma, Via di Val Cannuta, 247, 00166 Roma, Italy
Department of Animal Health and Bioscience University of Aarhus, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
* Author to whom correspondence should be addressed.
Received: 22 April 2010; in revised form: 14 May 2010 / Accepted: 31 May 2010 / Published: 1 June 2010
(This article belongs to the Special Issue Ochratoxins
Abstract: The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.
Keywords: ochratoxin A; α-tocopherol; DNA damage; fibroblasts; swine
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Fusi, E.; Rebucci, R.; Pecorini, C.; Campagnoli, A.; Pinotti, L.; Saccone, F.; Cheli, F.; Purup, S.; Sejrsen, K.; Baldi, A. Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts. Toxins 2010, 2, 1265-1278.
Fusi E, Rebucci R, Pecorini C, Campagnoli A, Pinotti L, Saccone F, Cheli F, Purup S, Sejrsen K, Baldi A. Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts. Toxins. 2010; 2(6):1265-1278.
Fusi, Eleonora; Rebucci, Raffaella; Pecorini, Chiara; Campagnoli, Anna; Pinotti, Luciano; Saccone, Francesca; Cheli, Federica; Purup, Stig; Sejrsen, Kristen; Baldi, Antonella. 2010. "Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts." Toxins 2, no. 6: 1265-1278.