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Discovery and Characterization of Proteins Associated with Aflatoxin-Resistance: Evaluating Their Potential as Breeding Markers
USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA
Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
USDA-ARS, Mississippi State University, MS, USA
International Institute of Tropical Agriculture, Ibadan, Nigeria
Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA
* Author to whom correspondence should be addressed.
Received: 28 February 2010; in revised form: 15 April 2010 / Accepted: 19 April 2010 / Published: 26 April 2010
Abstract: Host resistance has become a viable approach to eliminating aflatoxin contamination of maize since the discovery of several maize lines with natural resistance. However, to derive commercial benefit from this resistance and develop lines that can aid growers, markers need to be identified to facilitate the transfer of resistance into commercially useful genetic backgrounds without transfer of unwanted traits. To accomplish this, research efforts have focused on the identification of kernel resistance-associated proteins (RAPs) including the employment of comparative proteomics to investigate closely-related maize lines that vary in aflatoxin accumulation. RAPs have been identified and several further characterized through physiological and biochemical investigations to determine their causal role in resistance and, therefore, their suitability as breeding markers. Three RAPs, a 14 kDa trypsin inhibitor, pathogenesis-related protein 10 and glyoxalase I are being investigated using RNAi gene silencing and plant transformation. Several resistant lines have been subjected to QTL mapping to identify loci associated with the aflatoxin-resistance phenotype. Results of proteome and characterization studies are discussed.
Keywords: aflatoxin-resistance; maize resistance; proteomics; breeding markers
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MDPI and ACS Style
Brown, R.L.; Chen, Z.-Y.; Warburton, M.; Luo, M.; Menkir, A.; Fakhoury, A.; Bhatnagar, D. Discovery and Characterization of Proteins Associated with Aflatoxin-Resistance: Evaluating Their Potential as Breeding Markers. Toxins 2010, 2, 919-933.
Brown RL, Chen Z-Y, Warburton M, Luo M, Menkir A, Fakhoury A, Bhatnagar D. Discovery and Characterization of Proteins Associated with Aflatoxin-Resistance: Evaluating Their Potential as Breeding Markers. Toxins. 2010; 2(4):919-933.
Brown, Robert L.; Chen, Zhi-Yuan; Warburton, Marilyn; Luo, Meng; Menkir, Abebe; Fakhoury, Ahmad; Bhatnagar, Deepak. 2010. "Discovery and Characterization of Proteins Associated with Aflatoxin-Resistance: Evaluating Their Potential as Breeding Markers." Toxins 2, no. 4: 919-933.