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Toxins 2018, 10(6), 236; https://doi.org/10.3390/toxins10060236

Basics of Antibody Phage Display Technology

1
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark
2
IONTAS Ltd., Cambridgeshire CB22 3EG, United Kingdom
*
Author to whom correspondence should be addressed.
Received: 31 May 2018 / Revised: 7 June 2018 / Accepted: 8 June 2018 / Published: 9 June 2018
(This article belongs to the Special Issue Discovery of Antibodies and Novel Antivenoms against Envenoming)
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Abstract

Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins. View Full-Text
Keywords: antibody discovery; recombinant antivenom; phage display; M13 phage; toxinology antibody discovery; recombinant antivenom; phage display; M13 phage; toxinology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ledsgaard, L.; Kilstrup, M.; Karatt-Vellatt, A.; McCafferty, J.; Laustsen, A.H. Basics of Antibody Phage Display Technology. Toxins 2018, 10, 236.

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