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Toxins 2018, 10(1), 41; doi:10.3390/toxins10010041

Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics

1
College of plant protection, Agricultural University of Hebei, Baoding 071000, China
2
College of life science, Agricultural University of Hebei, Baoding 071000, China
*
Authors to whom correspondence should be addressed.
Received: 28 November 2017 / Revised: 27 December 2017 / Accepted: 11 January 2018 / Published: 12 January 2018
(This article belongs to the Section Plant Toxins)
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Abstract

α-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry. Sixteen protein spots were identified that showed reproducible changes in the expression of at least 2-fold when compared to the control. Among these 16 spots, three were up-regulated and 13 were down-regulated. The decreased expression of several proteins associated with energy production and carbon metabolism suggested that these processes were affected by α-terthienyl. To search for the candidate proteins in this screen, A. thaliana T-DNA mutants of the candidate proteins were used to test their susceptibility to α-terthienyl. Amongst the others, attkl1, a mutant of transketolase, exhibited a significantly lower sensitivity to α-terthienyl when hit compared with Col-0. Based on the identification of the proteins associated with the response to α-terthienyl by proteomics, a candidate target protein transketolase was identified. View Full-Text
Keywords: α-terthienyl; proteomics; herbicidal mechanism; transketolase α-terthienyl; proteomics; herbicidal mechanism; transketolase
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Zhao, B.; Huo, J.; Liu, N.; Zhang, J.; Dong, J. Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics. Toxins 2018, 10, 41.

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