Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics
Abstractα-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry. Sixteen protein spots were identified that showed reproducible changes in the expression of at least 2-fold when compared to the control. Among these 16 spots, three were up-regulated and 13 were down-regulated. The decreased expression of several proteins associated with energy production and carbon metabolism suggested that these processes were affected by α-terthienyl. To search for the candidate proteins in this screen, A. thaliana T-DNA mutants of the candidate proteins were used to test their susceptibility to α-terthienyl. Amongst the others, attkl1, a mutant of transketolase, exhibited a significantly lower sensitivity to α-terthienyl when hit compared with Col-0. Based on the identification of the proteins associated with the response to α-terthienyl by proteomics, a candidate target protein transketolase was identified. View Full-Text
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Zhao, B.; Huo, J.; Liu, N.; Zhang, J.; Dong, J. Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics. Toxins 2018, 10, 41.
Zhao B, Huo J, Liu N, Zhang J, Dong J. Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics. Toxins. 2018; 10(1):41.Chicago/Turabian Style
Zhao, Bin; Huo, Jingqian; Liu, Ning; Zhang, Jinlin; Dong, Jingao. 2018. "Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics." Toxins 10, no. 1: 41.
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