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Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene
Bio-Protection Research Centre, Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand
Biotechnology Research Institute, Qinghai Academy of Agriculture and Forestry, Xining, China
* Author to whom correspondence should be addressed.
Received: 13 November 2009; in revised form: 20 November 2009 / Accepted: 7 December 2009 / Published: 8 December 2009
Abstract: Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.
Keywords: aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight
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Cite This Article
MDPI and ACS Style
Bradshaw, R.E.; Feng, Z.; Schwelm, A.; Yang, Y.; Zhang, S. Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene. Toxins 2009, 1, 173-187.
Bradshaw RE, Feng Z, Schwelm A, Yang Y, Zhang S. Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene. Toxins. 2009; 1(2):173-187.
Bradshaw, Rosie E.; Feng, Zhilun; Schwelm, Arne; Yang, Yongzhi; Zhang, Shuguang. 2009. "Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene." Toxins 1, no. 2: 173-187.