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Toxins 2009, 1(2), 173-187; doi:10.3390/toxins1020173
Article

Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene

1,* , 1
,
1
,
2
 and
1
1 Bio-Protection Research Centre, Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand 2 Biotechnology Research Institute, Qinghai Academy of Agriculture and Forestry, Xining, China
* Author to whom correspondence should be addressed.
Received: 13 November 2009 / Revised: 20 November 2009 / Accepted: 7 December 2009 / Published: 8 December 2009
(This article belongs to the Special Issue Advances in Mycotoxin Research)
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Abstract

Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.
Keywords: aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Bradshaw, R.E.; Feng, Z.; Schwelm, A.; Yang, Y.; Zhang, S. Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene. Toxins 2009, 1, 173-187.

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