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Toxins 2009, 1(2), 173-187; doi:10.3390/toxins1020173
Article

Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene

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Received: 13 November 2009; in revised form: 20 November 2009 / Accepted: 7 December 2009 / Published: 8 December 2009
(This article belongs to the Special Issue Advances in Mycotoxin Research)
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Abstract: Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.
Keywords: aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Bradshaw, R.E.; Feng, Z.; Schwelm, A.; Yang, Y.; Zhang, S. Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene. Toxins 2009, 1, 173-187.

AMA Style

Bradshaw RE, Feng Z, Schwelm A, Yang Y, Zhang S. Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene. Toxins. 2009; 1(2):173-187.

Chicago/Turabian Style

Bradshaw, Rosie E.; Feng, Zhilun; Schwelm, Arne; Yang, Yongzhi; Zhang, Shuguang. 2009. "Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene." Toxins 1, no. 2: 173-187.


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