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Nutrients 2017, 9(7), 676; doi:10.3390/nu9070676

Comparable Performance Characteristics of Plasma Thiamine and Erythrocyte Thiamine Diphosphate in Response to Thiamine Fortification in Rural Cambodian Women

1
Bevital AS, Laboratoriebygget Bergen, 5021 Bergen, Norway
2
Food, Nutrition, and Health, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada
3
Department of Applied Human Nutrition, Mount Saint Vincent University, Halifax, NS B3M 2J6, Canada
4
Helen Keller International, Cambodia Country Office, Phnom Penh 12301, Cambodia
5
National Sub-Committee for Food Fortification, Ministry of Planning, Phnom Penh 12000, Cambodia
6
National Nutrition Programme, Maternal and Child Health Centre, Ministry of Health, Phnom Penh 12202, Cambodia
7
Department of Clinical Science, University of Bergen, 5021 Bergen, Norway
8
Laboratory of Clinical Biochemistry, Haukeland University Hospital, 5021 Bergen, Norway
9
Healthy Mothers, Babies, and Children, South Australian Health and Medical Research Institute, Adelaide 5000, Australia
*
Author to whom correspondence should be addressed.
Received: 7 April 2017 / Revised: 19 June 2017 / Accepted: 24 June 2017 / Published: 29 June 2017
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Abstract

Background: Traditionally, vitamin B1 status is assessed by a functional test measuring erythrocyte transketolase (ETK) activity or direct measurement of erythrocyte thiamine diphosphate (eThDP) concentration. However, such analyses are logistically challenging, and do not allow assessment of vitamin B1 status in plasma/serum samples stored in biobanks. Using a multiplex assay, we evaluated plasma concentrations of thiamine and thiamine monophosphate (TMP), as alternative, convenient measures of vitamin B1 status. Methods: We investigated the relationships between the established biomarker eThDP and plasma concentrations of thiamine and TMP, and compared the response of these thiamine forms to thiamine fortification using samples from 196 healthy Cambodian women (aged 18–45 years.). eThDP was measured by high performance liquid chromatography with fluorescence detection (HPLC-FLD) and plasma thiamine and TMP by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Plasma thiamine and TMP correlated significantly with eThDP at baseline and study-end (p < 0.05). Among the fortification groups, the strongest response was observed for plasma thiamine (increased by 266%), while increases in plasma TMP (60%) and eThDP (53%) were comparable. Conclusions: Plasma thiamine and TMP correlated positively with eThDP, and all thiamine forms responded significantly to thiamine intervention. Measuring plasma concentrations of thiamine forms is advantageous due to convenient sample handling and capacity to develop low volume, high-throughput, multiplex assays. View Full-Text
Keywords: thiamine (vitamin B1); direct biomarkers; thiamine monophospahte; erythrocyte thiamine diphosphate; plasma thiamine (vitamin B1); direct biomarkers; thiamine monophospahte; erythrocyte thiamine diphosphate; plasma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

McCann, A.; Midttun, Ø.; Whitfield, K.C.; Kroeun, H.; Borath, M.; Sophonneary, P.; Ueland, P.M.; Green, T.J. Comparable Performance Characteristics of Plasma Thiamine and Erythrocyte Thiamine Diphosphate in Response to Thiamine Fortification in Rural Cambodian Women. Nutrients 2017, 9, 676.

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