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Nutrients 2016, 8(4), 198; doi:10.3390/nu8040198

Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells

1
Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina
2
Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina
3
Institute of Immunological and Physiopathological Research (IIFP, CONICET-UNLP), School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina
4
Center of Experimental and Applied Endocrinology (CENEXA, UNLP-CONICET, PAHO/WHO Collaborating Center for Diabetes), La Plata Medical School, Universidad Nacional de La Plata, La Plata 1900, Argentina
*
Author to whom correspondence should be addressed.
Received: 20 January 2016 / Revised: 11 March 2016 / Accepted: 22 March 2016 / Published: 2 April 2016
(This article belongs to the Special Issue Diet and Metabolic Dysfunction)
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Abstract

We have previously addressed that fructose rich diet (FRD) intake for three weeks increases the adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue (RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolic parameters, number of adipocyte precursor cells (APCs) and in vitro adipogenic potential from control (CTR) and FRD adult male rats. Additionally, we have examined the direct fructose effects on the adipogenic capacity of normal APCs. FRD fed rats had increased plasma levels of insulin, triglyceride and leptin, and RPAT mass and adipocyte size. FACS studies showed higher APCs number and adipogenic potential in FRD RPAT pads; data is supported by high mRNA levels of competency markers: PPARγ2 and Zfp423. Complementary in vitro experiments indicate that fructose-exposed normal APCs displayed an overall increased adipogenic capacity. We conclude that the RPAT mass expansion observed in eight week-FRD fed rats depends on combined accelerated adipogenesis and adipocyte hypertrophy, partially due to a direct effect of fructose on APCs. View Full-Text
Keywords: retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zubiría, M.G.; Alzamendi, A.; Moreno, G.; Rey, M.A.; Spinedi, E.; Giovambattista, A. Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells. Nutrients 2016, 8, 198.

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