Next Article in Journal / Special Issue
The Intestinal Microbiota in Metabolic Disease
Previous Article in Journal
Letter to the Editor Re: Teoh SL et al., Nutrients 2016, 8, 57
Previous Article in Special Issue
Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
Article Menu

Export Article

Open AccessArticle
Nutrients 2016, 8(4), 203; doi:10.3390/nu8040203

Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle

1
Graduate School of Sport Sciences, Waseda University, 2-579-15, Mikajima, Tokorozawa city, Saitama 359-1192, Japan
2
Faculty of Sport Sciences, Waseda University, 2-579-15, Mikajima, Tokorozawa city, Saitama 359-1192, Japan
3
Institute of Advanced Active Aging Research, Waseda University, 2-579-15, Mikajima, Tokorozawa city, Saitama 359-1192, Japan
4
Department of Food Science and Nutrition, The University of Shiga Prefecture, 2500 Hassaka-Cho, Hikone city, Shiga 522-8533, Japan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 13 January 2016 / Revised: 25 March 2016 / Accepted: 30 March 2016 / Published: 6 April 2016
(This article belongs to the Special Issue Diet and Metabolic Dysfunction)
View Full-Text   |   Download PDF [1957 KB, uploaded 6 April 2016]   |  

Abstract

Long-term high-fat diet increases muscle mitochondrial enzyme activity and endurance performance. However, excessive calorie intake causes intra-abdominal fat accumulation and metabolic syndrome. The purpose of this study was to investigate the effect of an alternating day high-fat diet on muscle mitochondrial enzyme activities, protein content, and intra-abdominal fat mass in rats. Male Wistar rats were given a standard chow diet (CON), high-fat diet (HFD), or alternate-day high-fat diet (ALT) for 4 weeks. Rats in the ALT group were fed a high-fat diet and standard chow every other day for 4 weeks. After the dietary intervention, mitochondrial enzyme activities and protein content in skeletal muscle were measured. Although body weight did not differ among groups, the epididymal fat mass in the HFD group was higher than those of the CON and ALT groups. Citrate synthase and beta-hydroxyacyl CoA dehydrogenase activities in the plantaris muscle of rats in HFD and ALT were significantly higher than that in CON rats, whereas there was no difference between HFD and ALT groups. No significant difference was observed in muscle glycogen concentration or glucose transporter-4 protein content among the three groups. These results suggest that an alternate-day high-fat diet induces increases in mitochondrial enzyme activities and protein content in rat skeletal muscle without intra-abdominal fat accumulation. View Full-Text
Keywords: high-fat diet; alternate-day; mitochondria; skeletal muscle; rat high-fat diet; alternate-day; mitochondria; skeletal muscle; rat
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, X.; Higashida, K.; Kawamura, T.; Higuchi, M. Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle. Nutrients 2016, 8, 203.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top