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Nutrients 2015, 7(4), 2109-2124; doi:10.3390/nu7042109

Detection of Sialic Acid-Utilising Bacteria in a Caecal Community Batch Culture Using RNA-Based Stable Isotope Probing

1
AgResearch Ltd, Food Nutrition & Health Team, Food & Bio-based Products Group, Grasslands Research Centre, Private Bag 11008, Palmerston North 4442, New Zealand
2
Microbiology & Hygiene Group, Faculty of Medical and Life Sciences, Furtwangen University, 78054 Villingen-Schwenningen, Germany
3
Arla Strategic Innovation Centre, Roerdrumvej 2, 8220 Brabrand, Denmark
*
Author to whom correspondence should be addressed.
Received: 30 January 2015 / Accepted: 18 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Microbiome and Human Health)
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Abstract

Sialic acids are monosaccharides typically found on cell surfaces and attached to soluble proteins, or as essential components of ganglioside structures that play a critical role in brain development and neural transmission. Human milk also contains sialic acid conjugated to oligosaccharides, glycolipids, and glycoproteins. These nutrients can reach the large bowel where they may be metabolised by the microbiota. However, little is known about the members of the microbiota involved in this function. To identify intestinal bacteria that utilise sialic acid within a complex intestinal community, we cultured the caecal microbiota from piglets in the presence of 13C-labelled sialic acid. Using RNA-based stable isotope probing, we identified bacteria that consumed 13C-sialic acid by fractionating total RNA in isopycnic buoyant density gradients followed by 16S rRNA gene analysis. Addition of sialic acid caused significant microbial community changes. A relative rise in Prevotella and Lactobacillus species was accompanied by a corresponding reduction in the genera Escherichia/Shigella, Ruminococcus and Eubacterium. Inspection of isotopically labelled RNA sequences suggests that the labelled sialic acid was consumed by a wide range of bacteria. However, species affiliated with the genus Prevotella were clearly identified as the most prolific users, as solely their RNA showed significantly higher relative shares among the most labelled RNA species. Given the relevance of sialic acid in nutrition, this study contributes to a better understanding of their microbial transformation in the intestinal tract with potential implications for human health. View Full-Text
Keywords: Sialic acid; piglet; microbiota; RNA-SIP; Prevotella Sialic acid; piglet; microbiota; RNA-SIP; Prevotella
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Young, W.; Egert, M.; Bassett, S.A.; Bibiloni, R. Detection of Sialic Acid-Utilising Bacteria in a Caecal Community Batch Culture Using RNA-Based Stable Isotope Probing. Nutrients 2015, 7, 2109-2124.

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