Next Article in Journal
First Quantification of Calcium Intake from Calcium-Dense Dairy Products in Dutch Fracture Patients (The Delft Cohort Study)
Next Article in Special Issue
Epigenetic Mechanisms Underlying the Link between Non-Alcoholic Fatty Liver Diseases and Nutrition
Previous Article in Journal
The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats
Previous Article in Special Issue
Early Life Nutrition, Epigenetics and Programming of Later Life Disease
Article Menu

Export Article

Open AccessArticle
Nutrients 2014, 6(6), 2387-2403; doi:10.3390/nu6062387

DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome

1
Department of Nutrition, Food Science and Physiology, Center for Nutrition Research, University of Navarra, C/Irunlarrea 1, 31008-Pamplona, Spain
2
CIBER Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto de Salud Carlos III, Santiago de Compostela 15706, Spain
*
Author to whom correspondence should be addressed.
Received: 11 April 2014 / Revised: 11 June 2014 / Accepted: 16 June 2014 / Published: 23 June 2014
(This article belongs to the Special Issue Nutritional Epigenetics)
View Full-Text   |   Download PDF [422 KB, uploaded 23 June 2014]   |  

Abstract

Understanding the regulation of gene activities depending on DNA methylation has been the subject of much recent study. However, although polymorphisms of the HTR2A gene have been associated with both obesity and psychiatric disorders, the role of HTR2A gene methylation in these illnesses remains uncertain. The aim of this study was to evaluate the association of HTR2A gene promoter methylation levels in white blood cells (WBC) with obesity traits and depressive symptoms in individuals with metabolic syndrome (MetS) enrolled in a behavioural weight loss programme. Analyses were based on 41 volunteers (mean age 49 ± 1 year) recruited within the RESMENA study. Depressive symptoms (as determined using the Beck Depression Inventory), anthropometric and biochemical measurements were analysed at the beginning and after six months of weight loss treatment. At baseline, DNA from WBC was isolated and cytosine methylation in the HTR2A gene promoter was quantified by a microarray approach. In the whole-study sample, a positive association of HTR2A gene methylation with waist circumference and insulin levels was detected at baseline. Obesity measures significantly improved after six months of dietary treatment, where a lower mean HTR2A gene methylation at baseline was associated with major reductions in body weight, BMI and fat mass after the treatment. Moreover, mean HTR2A gene methylation at baseline significantly predicted the decrease in depressive symptoms after the weight loss treatment. In conclusion, this study provides newer evidence that hypermethylation of the HTR2A gene in WBC at baseline is significantly associated with a worse response to a weight-loss intervention and with a lower decrease in depressive symptoms after the dietary treatment in subjects with MetS. View Full-Text
Keywords: DNA methylation; HTR2A gene; obesity; metabolic syndrome; energy restriction; depressive symptoms; adiposity; epigenetics DNA methylation; HTR2A gene; obesity; metabolic syndrome; energy restriction; depressive symptoms; adiposity; epigenetics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Perez-Cornago, A.; Mansego, M.L.; Zulet, M.A.; Martinez, J.A. DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome. Nutrients 2014, 6, 2387-2403.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top