Next Article in Journal
DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome
Previous Article in Journal
Estimation of 24-Hour Urinary Sodium Excretion Using Spot Urine Samples
Nutrients 2014, 6(6), 2376-2386; doi:10.3390/nu6062376
Project Report

The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats

1,* , 2
, 3
, 4
, 5
, 6
 and 7
1 Department of Clinical Nutrition, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia 2 School of Medicine, University of Southampton, Southampton SO16 6YD, UK 3 Experimental Surgery Unit, KFMRC (King Fahd Medical Research Center), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia 4 Faculty of Medicine, Umm Al Qura University, Makkah 21955, Saudi Arabia 5 Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia 6 Pharmacology Unit, Biochemistry, Toxicology and Food deficiency Department, Animal Health Research Institute, Dokki, Gizza, Egypt 7 Surgical Registrar, University Hospital Southampton, Southampton SO16 6YD, UK
* Author to whom correspondence should be addressed.
Received: 13 March 2014 / Revised: 28 April 2014 / Accepted: 6 June 2014 / Published: 23 June 2014
View Full-Text   |   Download PDF [165 KB, uploaded 23 June 2014]

Abstract

We investigated the effect of extra virgin (EV) olive oil and genetically modified (GM) soybean on DNA, cytogenicity and some antioxidant enzymes in rodents. Forty adult male albino rats were used in this study and divided into four groups. The control group of rodents was fed basal ration only. The second group was given basal ration mixed with EV olive oil (30%). The third group was fed basal ration mixed with GM (15%), and the fourth group survived on a combination of EV olive oil, GM and the basal ration for 65 consecutive days. On day 65, blood samples were collected from each rat for antioxidant enzyme analysis. In the group fed on basal ration mixed with GM soyabean (15%), there was a significant increase in serum level of lipid peroxidation, while glutathione transferase decreased significantly. Interestingly, GM soyabean increased not only the percentage of micronucleated polychromatic erythrocytes (MPCE), but also the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PEC/NEC); however, the amount of DNA and NCE were significantly decreased. Importantly, the combination of EV olive oil and GM soyabean significantly altered the tested parameters towards normal levels. This may suggest an important role for EV olive oil on rodents’ organs and warrants further investigation in humans.
Keywords: soybean; olive oil; supplements; rats soybean; olive oil; supplements; rats
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

El-Kholy, T.A.; Hilal, M.A.; Al-Abbadi, H.A.; Serafi, A.S.; Al-Ghamdi, A.K.; Sobhy, H.M.; Richardson, J.R.C. The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats. Nutrients 2014, 6, 2376-2386.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert