- freely available
Nutrients 2013, 5(8), 3184-3211; doi:10.3390/nu5083184
Abstract: This review compares iron and zinc food sources, dietary intakes, dietary recommendations, nutritional status, bioavailability and interactions, with a focus on adults in economically-developed countries. The main sources of iron and zinc are cereals and meat, with fortificant iron and zinc potentially making an important contribution. Current fortification practices are concerning as there is little regulation or monitoring of intakes. In the countries included in this review, the proportion of individuals with iron intakes below recommendations was similar to the proportion of individuals with suboptimal iron status. Due to a lack of population zinc status information, similar comparisons cannot be made for zinc intakes and status. Significant data indicate that inhibitors of iron absorption include phytate, polyphenols, soy protein and calcium, and enhancers include animal tissue and ascorbic acid. It appears that of these, only phytate and soy protein also inhibit zinc absorption. Most data are derived from single-meal studies, which tend to amplify impacts on iron absorption in contrast to studies that utilize a realistic food matrix. These interactions need to be substantiated by studies that account for whole diets, however in the interim, it may be prudent for those at risk of iron deficiency to maximize absorption by reducing consumption of inhibitors and including enhancers at mealtimes.
Iron and zinc are the two most abundant trace minerals in the human body, with 3–4 g of iron and 1.5–2.5 g of zinc present in the average adult [1,2]. In human nutrition, zinc and iron are frequently assessed together [3,4,5,6,7,8,9] as these minerals share common dietary sources, the absorption of both nutrients from food is believed to be enhanced and inhibited by similar compounds [4,5,7,9], and consequently, deficiency of both nutrients is thought to occur simultaneously. This review aims to discuss iron and zinc in terms of dietary sources, bioavailability and dietary interactions drawing on literature from human studies. In addition, we have reviewed dietary recommendations for iron and zinc, current intakes and nutritional status in older adolescents and adults in English-speaking, economically-developed countries. This paper communicates the most recent information regarding iron and zinc recommendations, nutrition surveys and nutrition research.
Two electronic databases, PubMed and EbscoHost, were used to undertake literature searches for studies examining iron and zinc bioavailability, and interactions between iron and zinc. The searches were conducted between February 2012 and May 2013. The terms used to identify relevant studies are shown in Table 1 below.
|Search terms for iron and zinc bioavailability||Search terms for interactions between dietary iron and zinc|
|5||absorption and bioavailability||iron and zinc|
|6||iron and absorption and bioavailability||interaction and iron and zinc|
|7||zinc and absorption and bioavailability||supplementation and iron and zinc|
The searches were subsequently limited to studies published in the English language and those conducted in humans. In addition, reference lists of the retrieved original and review articles were searched to identify any other relevant studies.
Government publications were identified to determine current dietary recommendations for iron and zinc. National survey publications were identified to determine current iron and zinc intakes and status. Both types of publications were limited to data on older adolescents and adults in English-speaking, economically-developed countries.
For Figure 1, Release 25 of the US Department of Agriculture National Nutrient Database for Standard Reference was used to source the iron and zinc content of foods identified in national surveys as contributing to iron or zinc intakes. Efforts were made to identify fortified breakfast cereals that are marketed across English-speaking countries. Nutritional information published online by manufacturers was found for All-Bran® and Cheerios® in Australia and New Zealand, the US, and Canada, but not the UK. The iron and zinc content of foods was plotted using Minitab 15  and Spearman correlation tests were used to determine the associations between iron and zinc content according to whether foods are of plant origin, animal origin, or fortified with iron and zinc.
3. Dietary Sources and Intakes of Iron and Zinc
Foods recognized as good sources of iron tend to also contain zinc, the exception being dairy products which are a poor source of iron but recognized as a good source of zinc. Figure 1 depicts the association between iron content and zinc content in foods identified in national surveys as the main contributors of dietary iron or zinc intakes [11,12,13,14], as well as select fortified foods [15,16,17,18,19]. Using the foods in Figure 1 as an example, the association between iron and zinc content seems to be similar in foods of animal origin (r = 0.71, P = 0.001) and unfortified foods of plant origin (r = 0.68, P = 0.005).
The animal-derived foods presented in Figure 1 contain 189% more zinc than the unfortified plant-derived foods (mean 4.6 mg vs. 1.6 mg). Given this disparity, it is unsurprising that a recent meta-analysis found zinc intakes and zinc status to be lower among males and females following a vegetarian diet compared to those who consume meat . The iron content of the foods in Figure 1 are more comparable, with animal-derived foods containing 0.64% less iron than unfortified plant-derived foods (mean 3.0 mg vs. 3.1 mg). Iron intakes have been found to be similar in those following a vegetarian or omnivorous diet [6,22,23,24,25], however the iron status of vegetarians is often lower [22,23,25,26]. It is widely accepted that iron and perhaps zinc in animal-derived foods are more bioavailable than the iron and zinc present in plant-derived foods, although a well-planned vegetarian diet can provide sufficient amounts of dietary iron and zinc .
Government agencies in the US, Canada, the UK, New Zealand and Australia have assessed population dietary iron and zinc intakes, and the US, the UK, New Zealand and Australia have also assessed the main dietary sources contributing to these intakes (Table 2, Table 3). As foods have been categorized differently in these national surveys (e.g., whether pasta or rice have been grouped with breakfast cereals and bread), foods have been grouped into broader categories in Table 2, Table 3 for consistency. Across the surveys and among males and females, the bulk of dietary iron and zinc was sourced from cereal and meat products. Vegetables contributed to iron but not zinc intakes, and dairy contributed to zinc but not iron intakes. Males had higher intakes of both iron and zinc than females. Females were more likely to have inadequate iron intakes than males in all countries. In contrast, due to the higher recommendations for males, males were more likely than females to have inadequate zinc intakes in all countries but the US (Table 2, Table 3).
The contribution of cereal foods to iron and zinc intakes is noteworthy as these foods are often targets for fortification. In economically-developed countries, such fortification tends to be market-driven (e.g., voluntary fortification of breakfast cereals) or targeted-fortification (e.g., infant formula), as opposed to mass-fortification (e.g., mandatory fortification of staple foods such as wheat flour) . The balance of these three forms of iron and zinc fortification varies across countries. Mass-fortification of iron in wheat flour is mandatory in the US, Canada (each 44 ppm; began in the 1940s ) and the UK (16.5 ppm, began in 1953 ) but is not mandatory in Australia or New Zealand . Mass zinc fortification is not mandatory in any English-speaking, economically-developed countries, and is only mandatory in countries which are not a focus of this review: Indonesia (30 ppm in wheat flour), Jordan (20 ppm in wheat flour), Mexico (16 ppm in wheat and maize flours) and South Africa (15 ppm in wheat and maize flours) [31,32]. The breakfast cereals displayed in Figure 1 are an example of the variation in market-driven fortification worldwide. These cereals demonstrate that the same product around the world can vary markedly in the concentrations of fortificant iron and zinc. Importantly, as displayed in Figure 1, there is a tendency for the levels of fortificant iron and zinc in the cereals to exceed the levels naturally present in most foods.
There is also variance in the regulation of voluntarily fortified foods across countries. In Australia and New Zealand, only certain foods are permitted to be vectors for fortificant iron and zinc, and the amount of fortificant is indirectly limited through labeling and marketing restrictions . For example, manufacturers cannot claim more than 3.0 mg iron for every 50 g of bread , although there are no regulations preventing manufacturers from adding more than 3.0 mg per 50 g. Canada also has restrictions as to which foods may be fortified with iron and zinc, and in certain cases the Canadian regulations mandate the quantity that must be added to specific foods . For instance, if iron or zinc are added to a breakfast cereal, the cereal must contain 13.3 mg of iron and/or 3.5 mg of zinc per 100 g so that iron- or zinc-fortified cereals all contain the same concentration . While Canadian regulations stipulate upper limits on fortificant vitamin A, C, D, E and the B-group vitamins, there are no such limits for iron and zinc . The European Union expressly permits the use of iron and zinc as fortificants , although the maximum level of fortification is at the discretion of individual Member States . In the UK, market-driven fortification is not restricted by limits other than the food needing to be safe for human consumption [37,38]. The US also does not uphold limits of iron and zinc fortification. In the US, manufacturers are provided with guidelines for amounts of nutrients per 100 kcal based on the Recommended Dietary Allowance which they can choose to use . The worldwide lack of constraint or limits on the amount of fortificant iron and zinc permitted in foods is concerning as accumulation of iron in the body can lead to heart and liver failure  and habitual, moderately-high intakes of zinc (i.e., from 15 mg/day) may lead to impaired copper status, which may then adversely affect lipoprotein metabolism . The contribution of fortified foods to nutrient intakes worldwide is largely unknown, with the US’ Institute of Medicine calling for government and industry groups to collaboratively investigate the impact . Despite the lack of empirical evidence, the role of fortified foods must be considered as levels of fortification worldwide are unprecedented . Accordingly, the proportional contributions of foods presented in Table 2, Table 3 should be interpreted cautiously as information from Australia, the UK, and the US is over 10 years old and may not reflect the current abundance of market-driven fortified foods. Importantly, with the exception of New Zealand, there is a lack of national data from a single survey period showing the intakes, adequacy of the intakes, and the main food sources of iron or zinc.
4. Recommended Intakes of Iron and Zinc
Many countries have quantified the dietary requirements of humans. These reference values are generally determined by a panel based on scientific evidence and are used to assess whether the intakes of individuals or groups are adequate . For each nutrient, recommendations are derived from the frequency distribution of requirements of a group of individuals. The resulting values are an Estimated Average Requirement (EAR), which is generally the quantity meeting the needs of half the healthy individuals in a given life stage and sex group (i.e., the 50th percentile) . Another key reference value can be derived from the EAR, known as the Recommended Dietary Intake (RDI) in Australia and New Zealand, the Recommended Dietary Allowance (RDA) in the US and Canada, and the Reference Nutrient Intake (RNI) in the UK . This value meets the requirements of nearly all individuals in a given life stage and sex group. Across the US, Canada, the UK, New Zealand and Australia, the EAR for iron ranges from 5 to 11.4 mg/day and the EAR for zinc ranges from 5.5 to 12 mg/day (Table 4). The European Union is currently reviewing dietary recommendations with intent to standardize recommendations across all Member States . While recommendations vary across these economically-developed nations, there is consistency in that greater amounts of iron are recommended for women, and greater amounts of zinc are recommended for men.
|Country||Survey||Sample||Dietary iron intake (mg/day)||Inadequate intakes (i.e., intakes < EAR) %||Top 3 Food sources||Contribution of food to iron intake %|
|Australia||1995 National Nutrition Survey ||Males 19+ years (n = 5081)||Median: 15.2 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||41|
|Meat and meat products (includes poultry)||22|
|Females 19+ years (n = 5770)||Median: 11.1 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||38|
|Meat and meat products (includes poultry)||17|
|Canada||2004 Community Health Survey ||Males 19+ years (n = 8470)||Median (25%–75% IQR): 15.7 (12.9–19.1)||0.4 (SE 0.1)|
|Females 19+ years (n = 10350)||Median (25%–75% IQR): 11.9 (9.9–14.3)||19–30 years: 17 (SE 1.5)|
|31–50 years: 18 (SE 1.1)|
|51–70 years: <3 a|
|>70 years: 2 (SE 0.4)|
|New Zealand||2008/2009 Adult Nutrition Survey ||Males 15+ years (n = 2066)||Median (25%–75% IQR): 13.2 (12.8–13.6)||1 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||40|
|Meat and meat products (includes poultry)||20|
|Females 15+ years (n = 2655)||Median (25%–75% IQR): 9.9 (9.6–10.2)||10 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||36|
|Meat and meat products (includes poultry)||14|
|UK||Iron intake and inadequate intakes: 2008/09–2010/11 UK National Diet and Nutrition Survey |
Food sources: 2000–2001 National Diet and Nutrition Survey 
|Males 19–64 years (n = 346)||Median (lower 2.5 percentile-upper 2.5 percentile): 11.7 (5.7–22.3)||1 a,b||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||44|
|Meat and meat products (includes poultry)||19|
|Females 19–64 years (n = 461)||Median (lower 2.5 percentile-upper 2.5 percentile): 9.7 (4.0–16.3)||22 a,b||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||45|
|Meat and meat products (includes poultry)||15|
|US||Iron intake: NHANES 2009–2010 ||Iron intake:
Males 20+ years (n = 2789)
|Mean (SE): 17.5 (0.43)||Males 19+ years <3 a|
|Inadequate intakes: NHANES 2001–2002 ||Iron intake:
Females 20+ years (n = 2973)
|Mean (SE): 12.9 (0.33)||Females 19+ years 10 a|
|Food sources: NHANES 2003–2006 ||Food sources: Males and|
females 19+ years (n = 9490)
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||47|
|Meat and meat products (includes poultry)||10|
a No measure of spread or SE available; b Intakes below the Lower Reference Nutrient Intake (meets the requirements of 2.5% of the age and lifespan group).Abbreviations: NHANES—National Health and Nutrition Examination Survey; EAR—Estimated Average Requirement (meets the needs of 50% of individuals in the life stage group); IQR—interquartile range; SE—standard error.
|Country||Survey||Sample||Dietary zinc intake (mg/day)||Inadequate intakes (i.e., intakes < EAR) %||Top 3 Food sources||Contribution of food to zinc intake %|
|Australia||1995 National Nutrition Survey ||Males 19+ years (n = 5081)||Median: 14.4 a||Meat and meat products (includes poultry)||39|
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||24|
|Females 19+ years (n = 5770)||Median: 9.7 a||Meat and meat products (includes poultry)||32|
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||24|
|Canada||2004 Community Health Survey ||Males 19+ years (n = 8470)||Median (25%–75% IQR): 11.9 (10.3–13.8)||17 (SE 1.4)|
|Females 19+ years (n = 10350)||Median (25%–75% IQR): 9.5 (8.2–11.1)||14 (SE 1.3)|
|New Zealand||2008/2009 Adult Nutrition Survey ||Males 15+ years (n = 2066)||Median (25%–75% IQR): 12.9 (12.4–13.4)||39 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||33|
|Meat and meat products (includes poultry)||28|
|Females 15+ years (n = 2655)||Median (25%–75% IQR): 9.0 (8.7–9.3)||11 a||Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||30|
|Meat and meat products (includes poultry)||24|
|UK||Zinc intake and inadequate intakes:2008/09–2010/11 UK National Diet and Nutrition Survey |
Food sources:2000–2001 National Diet and Nutrition Survey 
|Males 19–64 years (n = 346)||Median (lower 2.5 percentile-upper 2.5 percentile): 9.6 (4.7–20.1)||8 a,b||Meat and meat products (includes poultry)||36|
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||25|
|Females 19–64 years (n = 461)||Median (lower 2.5 percentile-upper 2.5 percentile): 7.6 (3.7–13.1)||3 a,b||Meat and meat products (includes poultry)||30|
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||26|
|US||Zinc intake: NHANES 2009–2010 |
Inadequate intakes: NHANES 2001–2002 
Food sources: NHANES 2003–2006 
|Zinc intake:Males 20+ years (n = 2789)||Mean (SE): 14.2 (0.22)||Males 19+ years 11 (SE 1.0)|
|Zinc intake:Females 20+ years (n = 2973)||Mean (SE): 9.8 (0.17)||Females 19+ years 17 (SE 1.6)|
|Food sources: Males and females 19+ years (n = 9490)||Meat and meat products (includes poultry)||35|
|Cereals and cereal products (includes breads, breakfast cereals, biscuits, cakes)||15|
a No measure of spread or SE available; b Intakes below the Lower Reference Nutrient Intake (meets the requirements of 2.5% of the age and life stage group).Abbreviations: NHANES—National Health and Nutrition Examination Survey; EAR—Estimated Average Requirement (meets the needs of 50% of individuals in the life stage group); IQR—interquartile range; SE—standard error.
|Australia and New Zealand ||UK ||US and Canada |
|EAR (mg/day)||RDI (mg/day)||EAR (mg/day)||RNI (mg/day)||EAR (mg/day)||RDA (mg/day)|
|Iron||Males 19–50 years||6||8||6.7||8.7||6||8|
|Males 51+ years||6||8||6.7||8.7||6||8|
|Females 19–50 years a||8||18||11.4||14.8||8.1||18|
|Females 51+ years||5||8||6.7||8.7||5||8|
|Zinc||Males 19–50 years||12||14||7.3||9.5||9.4||11|
|Males 51+ years||12||14||7.3||9.5||9.4||11|
|Females 19–50 years a||6.5||8||5.5||7.0||6.8||8|
|Females 51+ years||6.5||8||5.5||7.0||6.8||8|
a Non-pregnant and non-lactating women.Abbreviations: EAR—Estimated Average Requirement (meets the needs of 50% of individuals in the life stage group); RDI/RNI/RDA—Recommended Dietary Intake/Reference Nutrient Intake/Recommended Dietary Allowance (meets the needs of nearly all individuals in the life stage group).
The chemical forms of iron and possibly zinc in food influence the amount absorbed through the gastrointestinal tract and into the bloodstream. In food, iron is present as heme iron and inorganic non-heme iron, with heme iron present exclusively in meats and non-heme iron present across the food chain in plants, eggs, milk, and meats . It is understood that the human body regulates iron status through absorption rather than secretion , thus absorption of heme and non-heme iron has been widely studied, but much remains unknown . Heme iron is readily absorbed through the small intestine, whereas non-heme iron is susceptible to being affected by compounds that impede or facilitate absorption and is less bioavailable . It has been suggested that zinc is found as organic complexes with protein in meats, and as inorganic salts in plant foods [54,55], however no absorption studies (i.e., using isotopic labeling) have been conducted in humans to determine whether these forms of zinc differ in bioavailability. In contrast to iron, the body regulates zinc homeostasis through gastrointestinal secretion and excretion of endogenous zinc in addition to absorption of exogenous zinc [43,56]. As zinc secretions are a major contributor to zinc status, it is possible interference with absorption has less of an impact on zinc status compared to iron, although research investigating this premise is required.
6. Inhibitors and Enhancers of Absorption
Iron and zinc are noted to interact with other meal components resulting in enhancement or inhibition of absorption through the intestine wall and into the bloodstream [57,58]. The absorption studies that have explored these interactions have generally tested absorption or fractional absorption from single meals using the widely-accepted stable isotopic or radioisotopic labeling techniques [56,59,60]. In these methods, stable isotopes or radioisotopes of iron or zinc are placed in a “carrier” meal (e.g., a bread roll or more complex food matrices including hamburger meals) that is consumed with or without a meal component suspected to influence absorption. To estimate iron absorption, incorporation of these stable isotopes or radioisotopes into erythrocytes or whole body counting of radioactivity is measured, while stable isotopes or radioisotopes in feces, urine, or the whole body are measured to estimate zinc absorption . The amount of iron or zinc absorbed in the presence of the meal component in question is then compared to the amount absorbed without that meal component. In general, participants act as their own control, consuming the isotopes with and without the meal component of interest. The majority of absorption research has investigated iron, in particular non-heme iron, and the meal components investigated include phytate, polyphenols, animal tissue, soy protein, calcium, and ascorbic acid.
Phytic acid (myo-inositol hexakisphosphate; IP6) and its salts (phytates) are the main storage form of phosphate in seeds and grains [62,63,64]. Chemically, phytic acid and phytate are known to be powerful chelators, binding to metals and forming insoluble compounds , and for this reason, it is thought phytate may impede absorption of nutrients such as iron and zinc. Single-meal studies of phytate in bran or as sodium phytate have demonstrated that as little as 2 mg phytate can reduce iron absorption by 18% . These studies have also shown that the inhibitory effect increases with the phytate content, such that 250 mg phytate in a meal can reduce absorption by 82% [65,66]. Furthermore, hydrolyzing phytate by dehulling grains, adding phytase or hydrochloric acid, or catalyzing the action of endogenous phytase (e.g., soaking bran for 24 h at 55 °C) minimizes the inhibition of iron absorption [66,67,68], with catalysis of endogenous phytase increasing absorption by 119% . There is also some evidence that other myo-inositol phosphates that contain fewer phosphate groups than IP6 (e.g., IP3, IP4, IP5) may also impede iron absorption . The influence of phytate on zinc absorption has not been as extensively investigated. Larsson et al.  and Fredlund et al.  have indicated phytate may inhibit zinc absorption, although the study by Fredlund et al. pooled the absorption data of all participants rather than using a stronger cross-over design . Thus, evidence for the inhibiting effect of phytate is weaker for zinc.
Absorption studies of phytate have tested doses of up to 250 mg phytate per meal. In a review of studies that have estimated phytate intakes around the world, Reddy  found omnivorous adult intakes to range from 395 to 1293 mg/day in the US and the UK . As small amounts of phytate can reduce iron absorption significantly, it is likely that dietary phytate does contribute to reduced iron absorption in Western diets, however interactions throughout the whole food matrix may temper the chelating strength of phytate . Although the findings of single-meal absorption studies may therefore not necessarily apply to a varied, whole diet, this premise has not been satisfactorily tested in a long-term dietary study. To investigate whether long-term consumption of phytate can affect iron status, Bach Kristensen et al. asked premenopausal women to consume high-fiber bread or dephytinized high-fiber bread for four months . However, women receiving the dephytinized bread in this study still received a considerable amount of phytate (daily serve of 300 g dephytinized bread contained approximately 456 mg vs. 549 mg phytate in non-dephytinized bread), and accordingly, there was no difference in iron status . A similar study design utilizing a more drastic reduction of phytate content may provide stronger evidence.
6.2. Polyphenols in Beverages
Polyphenols are a class of antioxidants, and complex combinations of polyphenols are found in foods and beverages sourced from plants including tea and coffee . Single-meal studies using tea and coffee as sources of polyphenols have shown that polyphenols decrease iron absorption [75,76,77,78,79]. Although few studies have measured the polyphenol content of test beverages, it appears polyphenols have a dose-dependent effect on iron absorption, with increasingly concentrated tea having more potency . Tea made from 3 g tea leaves concentrated at 396 mg/275 mL serve polyphenols reduced absorption by 91%, while the same tea diluted to 99 mg/275 mL serve polyphenols reduced iron absorption by 82% . The timing of beverage consumption may also be influential. This notion was demonstrated by Morck et al. , who found that consuming coffee one hour following a meal or simultaneously with a meal both inhibited iron absorption by 44%, while coffee consumption one hour prior to food had no effect. The specific polyphenolic compounds responsible for the decreased iron absorption have not all been identified, although the influence of catechin polyphenols present in these beverages (e.g., epigallocatechin-3-gallate) has been demonstrated in Caco-2 cell models of human iron absorption . While the inhibitory properties of polyphenols on iron absorption have consistently been demonstrated in absorption studies, complications arise when translating these findings into dietary recommendations. The quantity and composition of polyphenols varies widely, for example the total amount of catechins in tea made from 100 g of dry black tea may range from 1019 to 12,480 mg, and the processing of tea leaves, form (e.g., tea bag versus loose leaf) and brewing method (including amount and temperature of water and brew time) further affect the resulting content of polyphenols . Adding milk to beverages does not appear to meaningfully alter the inhibitory effect of polyphenols .
No isotopic zinc absorption studies have been conducted for beverage polyphenols. Ganji and Kies  have used zinc balance methodology to measure the effect of tea. Participants resided in a metabolic ward and all input and output of zinc was measured with or without the co-consumption of tea . Tea consumption did not affect zinc balance , however as the body uses endogenous intestinal secretions to regulate zinc metabolism, balance techniques do not provide an estimate of zinc absorption [56,61].
6.3. Animal Tissue
Findings from absorption studies suggest that different animal tissues have varied impacts on iron absorption [83,84]. Consuming animal tissue such as pork, lamb, beef, chicken or fish with meals seem to have comparable enhancing effects on iron absorption [83,84], while egg, casein and dairy foods have no such effect [84,85]. Furthermore, it appears that incorporating animal tissue in meals can counteract the inhibitory effect of phytate [86,87]. Consuming 196 g red kidney beans (227 mg phytate) with 100 g salmon has been shown to improve iron absorption by 48% . The quantity of animal protein seems to be critical: 50 g of pork increased iron absorption from a rice meal (220 mg phytate) by 44%, but no effect was observed when just 25 g of pork was incorporated into the meal . It is unknown whether the amount of protein required differs depending on the source animal. The inclusion of animal tissue, likely at least 50 g, to a meal is expected to improve iron absorption. However, a longer-term study comparing a self-selected five-day diet containing 222 g/day of meat, fish and poultry found no difference in iron absorption . Therefore, the exact quantity and type of animal protein required remains unknown.
The influence of animal tissue on zinc absorption has been assessed by Sandström et al. , who observed increased zinc absorption when chicken was combined with a meal, while fish, milk, cheese and beef had no effect. However, this study was limited as it was not a cross-over study where participants acted as their own control. Rather, the results for all participants were pooled, thus the true effect on zinc absorption is not known.
6.4. Soy Protein
Up to 30 g of soy protein has been demonstrated to be an inhibitor of both iron and zinc absorption in single-meal studies [90,91,92,93]. While the phytate present in soy products contributes to the inhibition of iron absorption, some interference with absorption remains when phytate is removed, suggesting other compounds in soy are likely to be implicated . The interactions between phytate, soy and zinc absorption are not yet understood .
Results from studies investigating the influence of calcium from food and supplements on iron absorption have been conflicting. Studies assessing the effect of calcium naturally present in dairy foods have produced mixed results. In single-meal studies, consuming 150 mL milk (165 mg calcium) with bread rolls inhibited iron absorption by 57% , while 250 mL milk , 150 g milk and 125 g yoghurt  with more complex meals (e.g., steak meal, hamburger meal) had no effect. In relatively longer absorption studies measuring iron absorption over five days, consumption of dairy products inhibited absorption when all meals were provided  but no effect was observed when diets were self-selected by participants . Single-meal and single-day studies indicate that supplemental or fortificant calcium has an inhibitory effect on heme and non-heme iron absorption [94,98,99,100], with as little as 40 mg fortificant calcium reducing absorption by 49% . The inhibitory effect of supplemental calcium demonstrated in these short-term absorption studies has not translated to impaired iron status, with consumption of supplemental calcium (1000–1200 mg/day calcium) for 12 weeks to six months having no impact on iron status [98,101].
6.6. Ascorbic Acid
Ascorbic acid has consistently been shown to enhance iron absorption when provided in single-meal studies as food sources [9,76,105,106] or in fortificant form [79,105]. Importantly, the improvement in iron absorption seems to vary depending on the composition of the meal, with 50 mg ascorbic acid (equivalent to approximately 100 g of fresh orange) increasing absorption by 61% from a hamburger meal and 164% from a pizza meal . Similarly, iron absorption from bread rolls containing as much as 250 mg phytate was improved with the addition of 50 mg ascorbic acid , however 100 mg of ascorbic acid added to a meal of rice and soup could not counteract the inhibitory effect of tea . The influence of meal composition and the whole food matrix may also explain why the dramatic improvements in iron absorption seen in single-meal studies were attenuated to a non-significant 35% improvement in a self-selected five-day diet study (247 mg/day ascorbic acid vs. 51 mg/day) . The importance of considering the whole food matrix in relation to iron absorption, rather than individual food components, has further been demonstrated in long-term studies that have assessed whether consumption of ascorbic acid can affect iron status: while having 164 mg ascorbic acid in fruit with a breakfast cereal containing 272 mg phytate as a daily breakfast meal improved iron status , having 113 mg ascorbic acid was not sufficient to overcome the inhibitory effect of nearly 3000 mg phytate each day, and did not affect iron status . Limited research suggests that other organic acids such as citric acid, malic acid and tartaric acid may also enhance iron absorption .
While evidence for the impact of ascorbic acid on iron absorption is strong, no studies have demonstrated any such effect on zinc absorption. Single-meal studies measuring zinc absorption with ascorbic acid naturally present in a food source  or added as a fortificant  found no enhancing or inhibiting effects.
7. Iron and Zinc Losses
Homeostatic regulation of iron and zinc differ, with iron being regulated through absorption and zinc being regulated primarily through secretion. As the body does not have a means to eliminate excess iron, absorption from the small intestine is tightly regulated by hepcidin. Hepcidin is a peptide hormone that is present in higher concentrations when body iron is replete . Higher concentrations of hepcidin prevent ingested iron from entering the bloodstream by trapping iron in enterocytes which are naturally shed every two days , thereby preventing body iron from escalating to dangerous levels. In comparison, endogenous (pancreatic, biliary and intestinal) secretions comprise the main route of zinc loss, with larger zinc intakes being balanced by larger zinc secretions [113,114]. The main routes of iron and zinc losses are outlined in Table 5. The losses of iron in menstrual blood and zinc in ejaculate account for the sex-specific differences in dietary recommendations presented in Table 4.
|Main routes of iron loss||Main routes of zinc loss|
|Urine (<1 mg/day)||Endogenous intestinal losses (0.5 to 3 mg/day)|
|Feces (from occult blood loss and shed enterocytes; 0.6 mg/day)||Urine (0.5 to 0.7 mg/day)|
|Menstrual blood (0.4 to 0.5 mg/day)||Skin cells (0.5 to 0.7 mg/day)|
|Skin cells and sweat (0.2 to 0.3 mg/day)||Semen (1 mg/ejaculate)|
Adapted from 1, 2, 51, 113.
8. Iron and Zinc Status
8.1. Iron Status
Serum ferritin reflects the level of iron stores in the body  and is often used as a marker of iron status in population surveys. Table 6 displays the mean serum ferritin concentrations measured in recent population surveys of economically-developed nations and the prevalence of adequate or inadequate concentrations. Varied age classifications and cut-off values for adequacy prevent direct comparisons between countries, although it is evident that compared to men, women have lower serum ferritin and are more likely to have suboptimal serum ferritin concentrations. In women, serum ferritin concentrations seem to increase with age in line with menopausal status (Table 6). In considering Canada and New Zealand, the only two nations that have categorized both iron intakes (Table 2) and iron status of males and females (Table 6), women, particularly women of childbearing age, are less likely to meet the EAR for iron and are at greater risk of low iron stores. It is likely that the prevalence of low iron stores is higher than indicated in Table 6. Ferritin is a positive acute-phase response protein that increases during inflammation, thus in a state of inflammation, it no longer reflects the size of iron stores . As only the New Zealand survey accounted for the effects of inflammation (i.e., excluding participants with high levels of inflammation), low iron stores may be masked by inflammation in the remaining surveys.
|Country||Survey||Sample||Ferritin (mean (95% CI) µg/L)||Low ferritin %|
|Canada||2009–2011 Canadian Health|
Measures Survey 
|Males 20–49 years a||164 (152–177) b||1 c|
|Males 50–64 years a||166 (148–187) b||1 c|
|Males 65–79 years a||155 (138–175) b||0 c|
|Females 20–49 years d||41 (38–44) b||9 c|
|Females 50–64 years d||85 (73–98) b||6 c|
|Females 65–79 years d||89 (74–107) b||6 c|
|New Zealand||2008/09 New Zealand Adult Nutrition Survey ||Males 15+ years (n = 1470)||177 (165–189) e||2 f|
|Females 15+ years (n = 1878)||79 (75–84) e||8 f|
|UK||2008/09–2010/11 UK National Diet and Nutrition Survey ||Males 19–64 years (n = 160)||155 e,g|
|Females 19–64 years (n = 231)||53 e,g|
|US||NHANES 2003–2006 ||Females 20–39 years h (n = 1780)||36.5 (33.5–39.9) b||Females 20–49 years (n = 2539):13 c|
|Females 40–49 years h (n = 759)||40.0 (34.5–46.3) b|
a Total n = 1684 Canadian males aged 20–79 years; b Geometric mean c Low ferritin defined as <15 μg/L;d Total n = 1975 Canadian females aged 20–79 years; e Arithmetic mean f Low ferritin defined as <12 μg/L;g No CI available h Ferritin not measured in adult males.Abbreviations: NHANES—National Health and Nutrition Examination Survey (mean ferritin was assessed in the 2005–2006 cycle; prevalence of low ferritin was assessed in the 2003–2006 cycle); CI—confidence interval.
8.2. Zinc Status
While ferritin is an accepted measure of iron stores, there is no equivalent biochemical marker of zinc status. The most commonly used marker of zinc status is serum or plasma zinc . Serum zinc reflects intakes of zinc but is maintained within a narrow range (12–15 µmol/L) and requires fasting blood samples for meaningful comparisons [119,120]. It is not considered a specific marker of zinc nutrition as circulating zinc is rapidly mobilized and turned over to meet tissue requirements . Moreover, no reference ranges for serum or plasma zinc concentrations have been established for individuals, further limiting the diagnostic use of circulating zinc levels. The International Zinc Nutrition Consultative Group has suggested lower cut-offs of serum zinc for use in population studies based on the 2.5th percentile of NHANES II (1976–1980) participants , however given the difficulties with measuring a marker present in such minute quantities and the logistics of enforcing fasting status for blood sampling in national surveys, few countries measured zinc concentrations. As presented in Table 7, the US measured serum zinc in NHANES II (1976–1980)  and the UK measured plasma zinc in the 2008/09–2010/11 National Diet and Nutrition Survey . Similar to serum ferritin, it appears males have higher zinc concentrations than females. Without an established biomarker, it is difficult to evaluate dietary zinc intakes in relation to biochemical status.
|Country||Survey||Sample||Mean zinc (μmol/L)||2.5th Percentile (μmol/L)|
|US||NHANES II1976–1980 [121,122]||Males 10–64 years|
AM fasting (n = 1767)
|Males 10–64 years|
AM non-fasting (n = 1750)
|Males 10–64 years|
PM (n = 2765)
|Females 10–70+ years|
AM fasting (n = 1576)
|Females 10–70+ years|
AM non-fasting (n = 1658)
|Females 10–70+ years|
PM (n = 2343)
|UK||2008/09–2010/11 UK National Diet and Nutrition Survey ||Males 19–64 years|
AM fasting (n = 160)
|Females 19–64 years|
AM fasting (n = 231)
a Serum zinc; geometric mean; b Plasma zinc; arithmetic mean.Abbreviations: NHANES—National Health and Nutrition Examination Survey.
8.3. Correlation between Iron and Zinc Status
The value of using serum or plasma zinc as a measure of zinc status in small research studies is debatable , however a handful of studies have assessed correlations between iron and zinc status using such measures. Two studies have found positive correlations between serum or plasma zinc and serum ferritin [7,123], while two other studies have found no such association, but using categorical analysis, have found serum zinc concentrations to be lower among individuals with all-cause anemia [124,125]. Another study did not report assessment of correlation between plasma zinc and serum ferritin, but using categorical analysis found that serum ferritin was lower among participants with lower serum zinc . Although evidence is limited, there appears to be a positive association between iron and zinc status, and as iron and zinc losses have different routes, this association may indicate a link between iron and zinc nutriture.
9. Effect of Iron Intake on Zinc Status and Zinc Absorption
It is often cited that serum zinc is decreased upon ingestion of over 25 mg elemental iron in a single dose, or when the molar ratio of iron content to zinc content in a meal or supplement is 2:1 or greater [3,127,128]. This notion is based on a series of studies that measured serum zinc hourly for four to six hours following ingestion of solutions or test foods (e.g., oysters) [127,129,130,131]. The applicability of these studies to balanced diets was later queried by Solomons  who pointed out that the molar ratio of iron to zinc in foods does not generally exceed 1:1, however these findings may have implications with the use of dietary supplements. Nevertheless, studies that have investigated the effect of long-term iron supplementation on serum zinc have yielded conflicting results. Some studies have found no effect of iron supplementation on serum zinc concentration (elemental iron dose ranging from 7.5 to 79 mg) [133,134,135,136,137], while others have found supplemental iron may impair serum zinc concentrations (elemental iron dose ranging from 18 to 60 mg) [138,139]. It should be noted that while some of these studies were conducted to assess zinc absorption, serum zinc data may not provide any meaningful information on zinc absorption but instead reflect redistribution of body zinc [140,141]. Absorption studies using isotope labeling have found that the iron to zinc molar ratio of meals has no bearing on the amount of zinc absorbed, and furthermore, that iron inhibits absorption of zinc when both are tested in solution, but this effect is diminished when food matrices are used [142,143,144]. Accordingly, it appears the iron concentration of foods does not affect zinc absorption, and the effect of ingesting supplementary iron on zinc status is unknown.
10. Effect of Zinc Intake on Iron Status and Iron Absorption
Studies have also investigated the reverse relationship of whether zinc intake can affect iron status, with some studies finding zinc supplementation (dose ranging from 10 to 25 mg/day elemental zinc taken from six weeks to six months) leads to a decrease in serum or plasma ferritin ranging from 18% to 69% [145,146,147,148], while others have found serum ferritin to increase by 45% to 51% [149,150]. However, a meta-analysis exploring an array of effects of zinc supplementation in children found zinc supplementation does not have an effect on serum or plasma ferritin, although the authors acknowledged significant heterogeneity among studies .
The few studies that have used isotope labeling to measure iron absorption in relation to zinc intake have yielded mixed results. Zinc has been found to inhibit absorption of iron when the micronutrients are ingested in solution [152,153,154], but the same effect has not been demonstrated in a food matrix [152,153]. The long-term effect of zinc supplementation on iron absorption is still unclear. Studies have observed no change in iron absorption from a solution before and after supplementation (two months of 20 mg/day elemental zinc) , as well as an increase in iron absorption from food of 97% following supplementation (six weeks of 22 mg/day elemental zinc) . Similar to the effect of iron on zinc absorption, there is no evidence that consumption of zinc in foods affects iron absorption. Additionally, given the conflicting findings of existing research, the impact of supplementary zinc on iron status is yet to be determined.
The effects of iron intake on zinc status or zinc intake on iron status have not been consistently demonstrated. Meat, seafood, legumes and cereals tend to be sources of both dietary iron and dietary zinc. The contribution of cereals to intakes is noteworthy as cereal products are commonly fortified with iron and zinc. There is little regulation on such voluntary fortification practices, and few population nutrition surveys may be recent enough to reflect the current and unprecedented levels of fortification. Given the adverse consequences of excess iron and zinc consumption, assessment of the contribution of fortified foods to intakes is required. Across economically-developed nations, dietary recommendations for iron are higher in women and dietary recommendations for zinc are higher in men, reflecting the routes of loss. Across the same nations, women have tended to present with poorer iron and zinc status and are more likely to have inadequate iron stores. Despite claims that certain compounds inhibit and enhance absorption of both iron and zinc, there is insufficient evidence reflecting free-living populations to derive dietary recommendations, particularly in the case of zinc. Single-meal absorption studies have demonstrated that phytate, polyphenols in beverages, supplemental calcium and soy protein can inhibit iron absorption. Of these potential inhibitors, phytate and soy protein have been shown to also inhibit zinc absorption. Animal tissue and ascorbic acid have been found to enhance absorption of iron, however the effect of animal tissue on zinc absorption has not been convincingly tested and it appears ascorbic acid does not have an impact on zinc absorption. While single-meal studies have indicated that consumption of these compounds has a dramatic impact on iron absorption, these results are attenuated in longer-term absorption studies, and there is no consistent evidence that consumption of these compounds affects iron status. There is a lack of longer-term zinc absorption studies and studies assessing the impact of these compounds on zinc status.
Finally, it must be noted that discussion of national data was specific to English-speaking countries and these data may not be applied to other nations. Furthermore, animal models and in vitro studies have not been discussed, although these may provide further insight into iron and zinc nutriture. Despite the limitations of this review, topics for further research have been identified. Potential interactions need to be substantiated by studies investigating mixed diets in free-living samples, and these studies need to account for complex food matrices. Studies investigating whether iron from supplements inhibits zinc absorption or whether supplemental zinc inhibits iron absorption have been inconclusive, and no effects have been demonstrated in a food matrix. Further research is required to clarify the potential interactions between iron and zinc and other food components. Identification of a specific marker of zinc nutrition would advance understanding of dietary zinc, and research that integrates whole, varied diets with biomarker information is likely to indicate whether the dietary interactions involving iron and zinc have any influence on iron and zinc status. In the absence of such evidence, it may be prudent for individuals at risk of iron deficiency to maximize iron absorption by consuming potential absorption enhancers while avoiding inhibitors at mealtimes.
Karen Lim is supported by a Deakin University Postgraduate Research Scholarship.
Conflict of Interest
The authors declare no conflict of interest.
- King, J.; Cousins, R. Zinc. In Modern Nutrition in Health and Disease, 10th ed.; Shils, M., Ed.; Lippincott Williams & Wilkins: Philadelphia, PA, USA, 2006; pp. 271–285. [Google Scholar]
- Wood, R.; Ronnenberg, A. Iron. In Modern Nutrition in Health and Disease, 10th ed.; Shils, M., Ed.; Lippincott Williams & Wilkins: Philadelphia, PA, USA, 2006; pp. 248–270. [Google Scholar]
- Kordas, K.; Stoltzfus, R.J. New evidence of iron and zinc interplay at the enterocyte and neural tissues. J. Nutr. 2004, 134, 1295–1298. [Google Scholar]
- Sandstead, H.H. Causes of iron and zinc deficiencies and their effects on brain. J. Nutr. 2000, 130, 347S–349S. [Google Scholar]
- Hunt, J.R. Moving toward a plant-based diet: Are iron and zinc at risk? Nutr. Rev. 2002, 60, 127–134. [Google Scholar]
- Donovan, U.M.; Gibson, R.S. Iron and zinc status of young women aged 14 to 19 years consuming vegetarian and omnivorous diets. J. Am. Coll. Nutr. 1995, 14, 463–472. [Google Scholar]
- Gibson, R.; Heath, A.L.; Prosser, N.; Parnell, W.; Donovan, U.; Green, T.; McLaughlin, K.; O’Connor, D.; Bettger, W.; Skeaff, C. Are Young Women with Low Iron Stores at Risk of Zinc as Well as Iron Deficiency? In Trace Elements in Man and Animals 10; Roussel, A.M., Favier, A.E., Anderson, R.A., Eds.; Kluwer Academic Publishers: New York, NY, USA, 1999; pp. 323–328. [Google Scholar]
- Hunt, J.R. Bioavailability of iron, zinc, and other trace minerals from vegetarian diets. Am. J. Clin. Nutr. 2003, 78, 633S–639S. [Google Scholar]
- Nair, K.M.; Brahmam, G.N.; Radhika, M.S.; Dripta, R.C.; Ravinder, P.; Balakrishna, N.; Chen, Z.; Hawthorne, K.M.; Abrams, S.A. Inclusion of guava enhances non-heme iron bioavailability but not fractional zinc absorption from a rice-based meal in adolescents. J. Nutr. 2013, 143, 852–858. [Google Scholar]
- Minitab, Minitab 15. State College, Pennsylvania, PA, USA, 2007.
- McLennan, W.; Podger, A. National Nutrition Survey: Nutrient Intakes and Physical Measurements, Australia, 1995; Australian Bureau of Statistics: Canberra, Australia, 1998. [Google Scholar]
- University of Otago and Ministry of Health, A Focus on Nutrition: Key Findings of the 2008/09 New Zealand Adult Nutrition Survey; Ministry of Health: Wellington, New Zealand, 2011.
- Henderson, L.; Irving, K.; Gregory, J.; Bates, C.; Prentice, A.; Perks, J.; Swan, G.; Farron, M. The National Diet and Nutrition Survey: Adults Aged 19 to 64 Years—Vitamin and Mineral Intake and Urinary Analytes; The Stationery Office: London, UK, 2003. [Google Scholar]
- O’Neil, C.E.; Keast, D.R.; Fulgoni, V.L.; Nicklas, T.A. Food sources of energy and nutrients among adults in the US: NHANES 2003–2006. Nutrients 2012, 4, 2097–2120. [Google Scholar]
- Kellogg Canada All-Bran® Original Cereal, 2012. Available online: http://www.all-bran.ca/en/cereal/All-Bran_Original_cereal.aspx (accessed on 24 May 2013).
- Kellogg Company Kellogg (Aust) All-Bran® Original Cereal, 2012. Available online: http://www.all-bran.com.au/products/all-bran-original.aspx (accessed on 24 May 2013).
- Kellogg NA Co Kellogg’s® All-Bran® Original Cereal, 2013. Available online: http://www.all-bran.com/products/original-cereal.aspx (accessed on 24 May 2013).
- General Mills Cheerios®, 2013. Available online: http://www.cheerios.com/Products/Cheerios#.UZ63DrT4V8E (accessed on 24 May 2013).
- General Mills (Canada) Cheerios®, 2012. Available online: http://www.generalmillsfoodservice.ca/en/pdf/pos/CHEERIOS_ENG.pdf (accessed on 24 May 2013).
- USDA National Nutrient Database for Standard Reference; Release 25. 2012. Available online: http://www.ars.usda.gov/ba/bhnrc/ndl (accessed 20 April 2013).
- Foster, M.; Chu, A.; Petocz, P.; Samman, S. Effect of vegetarian diets on zinc status: A systematic review and meta-analysis of studies in humans. J. Sci. Food Agric. 2013, 93, 2362–2371. [Google Scholar]
- Ball, M.J.; Bartlett, M.A. Dietary intake and iron status of Australian vegetarian women. Am. J. Clin. Nutr. 1999, 70, 353–358. [Google Scholar]
- Reddy, S.; Sanders, T.A. Haematological studies on pre-menopausal Indian and Caucasian vegetarians compared with Caucasian omnivores. Br. J. Nutr. 1990, 64, 331–338. [Google Scholar]
- Worthington-Roberts, B.S.; Breskin, M.W.; Monsen, E.R. Iron status of premenopausal women in a university community and its relationship to habitual dietary sources of protein. Am. J. Clin. Nutr. 1988, 47, 275–279. [Google Scholar]
- Deriemaeker, P.; Aerenhouts, D.; de Ridder, D.; Hebbelinck, M.; Clarys, P. Health aspects, nutrition and physical characteristics in matched samples of institutionalized vegetarian and non-vegetarian elderly (>65years). Nutr. Metab. 2011, 8, 37. [Google Scholar]
- Li, D.; Sinclair, A.J.; Mann, N.J.; Turner, A.; Ball, M.J. Selected micronutrient intake and status in men with differing meat intakes, vegetarians and vegans. Asia Pac. J. Clin. Nutr. 2000, 9, 18–23. [Google Scholar]
- American Dietetic Association. Dietitians of Canada. Position of the American Dietetic Association and Dietitians of Canada: Vegetarian diets. J. Am. Diet Assoc. 2003, 103, 748–765.
- Allen, L.; de Benoist, B.; Dary, O.; Hurrell, R. Guidelines on Food Fortification with Micronutrients; World Health Organisation/Food and Agricultural Organisation: Geneva, Switzerland, 2006. [Google Scholar]
- Institute of Medicine, Food and Nutrition Board, Dietary Reference Intakes: Guiding Principles for Nutrition Labeling and Fortification; National Academies Press: Washington, DC, USA, 2003.
- Scientific Advisory Committee on Nutrition, Iron and Health; The Stationery Office: London, UK, 2010.
- Flour Fortification Initiative. Flour Fortification Initiative: Country Profiles, 2012. Available online: http://www.ffinetwork.org/country_profiles/index.php (accessed on 1 March 2013).
- Brown, K.H.; Hambidge, K.M.; Ranum, P. Zinc fortification of cereal flours: Current recommendations and research needs. Food Nutr. Bull. 2010, 31, S62–S74. [Google Scholar]
- Food Standards Australia New Zealand. Australia New Zealand Food Standards Code—Standard 1.3.2: Vitamins and Minerals, 2013. Available online: http://www.comlaw.gov.au/Details/F2013C00099 (accessed on 26 April 2013).
- Health Canada. Food and Drug Regulations, 2013. Available online: http://laws-lois.justice.gc.ca/PDF/C.R.C.,_c._870.pdf (accessed on 26 April 2013).
- Commission of the European Communities, Regulation (EC). No. 1925/2006 of the European Parliament and of the Council of 20 December 2006 on the addition of vitamins and minerals and of certain other substances to foods. Off. J. Eur. Union 2006, 404, 26–38.
- Schwitters, B.; Achanta, G.; van der Vlies, D.; Bast, A.; Hanekamp, J.C. The European regulation of food supplements and food fortification. Environ. Law. Manag. 2007, 19, 19–29. [Google Scholar]
- Food Standards Agency. Safe Upper Levels for Vitamins and Minerals. Report of Expert Group on Vitamins and Minerals, 2003. Available online: http://www.food.gov.uk/multimedia/pdfs/vitmin2003.pdf (accessed on 26 April 2013).
- UK Parliament. Food Safety Act, 1990. Available online: http://www.legislation.gov.uk/ukpga/1990/16/contents (accessed on 26 April 2013).
- Kohgo, Y.; Ikuta, K.; Ohtake, T.; Torimoto, Y.; Kato, J. Body iron metabolism and pathophysiology of iron overload. Int. J. Hematol. 2008, 88, 7–15. [Google Scholar]
- Fosmire, G.J. Zinc toxicity. Am. J. Clin. Nutr. 1990, 51, 225–227. [Google Scholar]
- Lawrence, M. Food Fortification: The Evidence, Ethics, and Politics of Adding Nutrients to Food; Oxford University Press: Oxford, UK, 2013. [Google Scholar]
- Lawrence, M.; Robertson, A. Reference Standards and Guidelines. In Public Health Nutrition—From Principle to Practice; Lawrence, M., Worsley, T., Eds.; Allen & Unwin: Crows Nest, Australia, 2007; pp. 39–70. [Google Scholar]
- Institute of Medicine, Food and Nutrition Board, Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc; National Academies Press: Washington, DC, USA, 2001.
- Dhonukshe-Rutten, R.A.; Timotijevic, L.; Cavelaars, A.E.; Raats, M.M.; de Wit, L.S.; Doets, E.L.; Tabacchi, G.; Roman, B.; Ngo-de la Cruz, J.; Gurinovic, M.; et al. European micronutrient recommendations aligned: A general framework developed by EURRECA. Eur. J. Clin. Nutr. 2010, 64, S2–S10. [Google Scholar]
- Statistics Canada, Canadian Community Health Survey Cycle 2.2, Nutrition (2004); Minister of Health Canada: Ottawa, Canada, 2009.
- UK Department of Health. National Diet and Nutrition Survey: Headline Results from Years 1 and 2 (Combined) of the Rolling Programme, 2008/09–2009/10; Headline Results from Years 1 and 2 (Combined) Tables, 2011. Available online: https://www.gov.uk/government/ uploads/system/uploads/attachment_data/file/152237/dh_128556.pdf (accessed on 27 April 2013).
- 2009–2010 What We Eat In America, NHANES Tables 1–40, 2010. Available online: http://www.ars.usda.gov/SP2UserFiles/Place/12355000/pdf/0910/tables_1–40_2009–2010.pdf (accessed on 29 April 2013).
- Moshfegh, A.; Goldman, J.; Cleveland, L. What We Eat in America, NHANES 2001–2002: Usual Nutrient Intakes From Food Compared to Dietary Reference Intakes; US Department of Agriculture, Agricultural Research Service: Washington, DC, USA, 2005. [Google Scholar]
- National Health and Medical Research Council, Nutrient Reference Values for Australia and New Zealand Including Recommended Dietary Intakes; National Health and Medical Research Council: Canberra, Australia, 2006.
- Department of Health, Dietary Reference Values for Food Energy and Nutrients for the United Kingdom; Her Majesty’s Stationary Office: London, UK, 1991.
- Gibson, R.S. Principles of Nutritional Assessment, 2nd ed.; Oxford University Press: New York, NY, USA, 2005. [Google Scholar]
- Knutson, M.D. Iron-sensing proteins that regulate hepcidin and enteric iron absorption. Annu. Rev. Nutr. 2010, 30, 149–171. [Google Scholar]
- Conrad, M.E.; Umbreit, J.N. Iron absorption and transport—An update. Am. J. Hematol. 2000, 64, 287–298. [Google Scholar]
- Gibson, R.S. The role of diet- and host-related factors in nutrient bioavailability and thus in nutrient-based dietary requirement estimates. Food Nutr. Bull. 2007, 28, S77–S100. [Google Scholar]
- Solomons, N.W.; Jacob, R.A.; Pineda, O.; Viteri, F. Studies on the bioavailability of zinc in man. II. Absorption of zinc from organic and inorganic sources. J. Lab. Clin. Med. 1979, 94, 335–343. [Google Scholar]
- Sandström, B. Bioavailability of zinc. Eur. J. Clin. Nutr. 1997, 51, S17–S19. [Google Scholar]
- Lönnerdal, B. Dietary factors influencing zinc absorption. J. Nutr. 2000, 130, 1378S–1383S. [Google Scholar]
- Hurrell, R.; Egli, I. Iron bioavailability and dietary reference values. Am. J. Clin. Nutr. 2010, 91, 1461S–1467S. [Google Scholar] [CrossRef]
- Layrisse, M.; Martínez-Torres, C. Model for measuring dietary absorption of heme iron: Test with a complete meal. Am. J. Clin. Nutr. 1972, 25, 401–411. [Google Scholar]
- Cook, J.D.; Layrisse, M.; Martinez-Torres, C.; Walker, R.; Monsen, E.; Finch, C.A. Food iron absorption measured by an extrinsic tag. J. Clin. Investig. 1972, 51, 805–815. [Google Scholar]
- Fairweather-Tait, S.J.; Dainty, J. Use of stable isotopes to assess the bioavailability of trace elements: A review. Food Addit. Contam. 2002, 19, 939–947. [Google Scholar]
- Cosgrove, D. The chemistry and biochemistry of inositol phosphates. Rev. Pure Appl. Chem. 1966, 16, 209–224. [Google Scholar]
- Reddy, N.; Sathe, S. Introduction. In Food Phytates; Reddy, N.R., Sathe, S., Eds.; CRC Press: Boca Raton, FL, USA, 2001; pp. 1–6. [Google Scholar]
- Shears, S.; Turner, B. Nomenclature and Terminology of Inositol Phosphates: Clarification and a Glossary of Terms. In Inositol Phosphates. Linking Agriculture and the Environment; Turner, B., Richardson, A., Mullaney, E., Eds.; CAB International: Wallingford, England, UK, 2007; pp. 1–6. [Google Scholar]
- Hallberg, L.; Brune, M.; Rossander, L. Iron absorption in man: Ascorbic acid and dose-dependent inhibition by phytate. Am. J. Clin. Nutr. 1989, 49, 140–144. [Google Scholar]
- Hallberg, L.; Rossander, L.; Skanberg, A. Phytates and the inhibitory effect of bran on iron absorption in man. Am. J. Clin. Nutr. 1987, 45, 988–996. [Google Scholar]
- Hurrell, R.F.; Reddy, M.B.; Juillerat, M.A.; Cook, J.D. Degradation of phytic acid in cereal porridges improves iron absorption by human subjects. Am. J. Clin. Nutr. 2003, 77, 1213–1219. [Google Scholar]
- Larsson, M.; Rossander-Hulthen, L.; Sandstrom, B.; Sandberg, A.S. Improved zinc and iron absorption from breakfast meals containing malted oats with reduced phytate content. Br. J. Nutr. 1996, 76, 677–688. [Google Scholar]
- Sandberg, A.S.; Brune, M.; Carlsson, N.G.; Hallberg, L.; Skoglund, E.; Rossander-Hulthen, L. Inositol phosphates with different numbers of phosphate groups influence iron absorption in humans. Am. J. Clin. Nutr. 1999, 70, 240–246. [Google Scholar]
- Fredlund, K.; Isaksson, M.; Rossander-Hulthen, L.; Almgren, A.; Sandberg, A.S. Absorption of zinc and retention of calcium: Dose-dependent inhibition by phytate. J. Trace Elem. Med. Biol. 2006, 20, 49–57. [Google Scholar]
- Reddy, N.R. Occurrence, Distribution, Content, and Dietary Intake of Phytate. In Food Phytates; Reddy, N.R., Sathe, S.K., Eds.; CRC Press: Boca Raton, FL, USA, 2001; pp. 25–52. [Google Scholar]
- Weaver, C.; Kannan, S. Phytate and Mineral Bioavailability. In Food Phytates; Reddy, N.R., Sathe, S.K., Eds.; CRC Press: Boca Raton, USA, 2001; pp. 211–219. [Google Scholar]
- Bach Kristensen, M.; Tetens, I.; Alstrup Jørgensen, A.B.; Dal Thomsen, A.; Milman, N.; Hels, O.; Sandström, B.; Hansen, M. A decrease in iron status in young healthy women after long-term daily consumption of the recommended intake of fibre-rich wheat bread. Eur. J. Nutr. 2005, 44, 334–340. [Google Scholar]
- Manach, C.; Scalbert, A.; Morand, C.; Remesy, C.; Jimenez, L. Polyphenols: Food sources and bioavailability. Am. J. Clin. Nutr. 2004, 79, 727–747. [Google Scholar]
- Disler, P.B.; Lynch, S.R.; Charlton, R.W.; Torrance, J.D.; Bothwell, T.H.; Walker, R.B.; Mayet, F. The effect of tea on iron absorption. Gut 1975, 16, 193–200. [Google Scholar]
- Hallberg, L.; Rossander, L. Effect of different drinks on the absorption of non-heme iron from composite meals. Hum. Nutr. Appl. Nutr. 1982, 36, 116–123. [Google Scholar]
- Hurrell, R.F.; Reddy, M.; Cook, J.D. Inhibition of non-haem iron absorption in man by polyphenolic-containing beverages. Br. J. Nutr. 1999, 81, 289–295. [Google Scholar]
- Morck, T.A.; Lynch, S.R.; Cook, J.D. Inhibition of food iron absorption by coffee. Am. J. Clin. Nutr. 1983, 37, 416–420. [Google Scholar]
- Thankachan, P.; Walczyk, T.; Muthayya, S.; Kurpad, A.V.; Hurrell, R.F. Iron absorption in young Indian women: The interaction of iron status with the influence of tea and ascorbic acid. Am. J. Clin. Nutr. 2008, 87, 881–886. [Google Scholar]
- Kim, E.Y.; Ham, S.K.; Shigenaga, M.K.; Han, O. Bioactive dietary polyphenolic compounds reduce nonheme iron transport across human intestinal cell monolayers. J. Nutr. 2008, 138, 1647–1651. [Google Scholar]
- Peterson, J.; Dwyer, J.; Bhagwat, S.; Haytowitz, D.; Holden, J.; Eldridge, A.; Beecher, G.; Aladesanmi, J. Major flavonoids in dry tea. J. Food Compost. Anal. 2005, 18, 487–501. [Google Scholar]
- Ganji, V.; Kies, C.V. Zinc bioavailability and tea consumption: Studies in healthy humans consuming self-selected and laboratory-controlled diets. Plant Foods Hum. Nutr. 1994, 46, 267–276. [Google Scholar]
- Bjorn-Rasmussen, E.; Hallberg, L. Effect of animal proteins on the absorption of food iron in man. Nutr. Metab. 1979, 23, 192–202. [Google Scholar]
- Cook, J.D.; Monsen, E.R. Food iron absorption in human subjects. III. Comparison of the effect of animal proteins on nonheme iron absorption. Am. J. Clin. Nutr. 1976, 29, 859–867. [Google Scholar]
- Hurrell, R.F.; Lynch, S.R.; Trinidad, T.P.; Dassenko, S.A.; Cook, J.D. Iron absorption in humans as influenced by bovine milk proteins. Am. J. Clin. Nutr. 1989, 49, 546–552. [Google Scholar]
- Navas-Carretero, S.; Perez-Granados, A.M.; Sarria, B.; Carbajal, A.; Pedrosa, M.M.; Roe, M.A.; Fairweather-Tait, S.J.; Vaquero, M.P. Oily fish increases iron bioavailability of a phytate rich meal in young iron deficient women. J. Am. Coll. Nutr. 2008, 27, 96–101. [Google Scholar]
- Bæch, S.B.; Hansen, M.; Bukhave, K.; Jensen, M.; Sørensen, S.S.; Kristensen, L.; Purslow, P.P.; Skibsted, L.H.; Sandström, B. Nonheme-iron absorption from a phytate-rich meal is increased by the addition of small amounts of pork meat. Am. J. Clin. Nutr. 2003, 77, 173–179. [Google Scholar]
- Reddy, M.B.; Hurrell, R.F.; Cook, J.D. Meat consumption in a varied diet marginally influences nonheme iron absorption in normal individuals. J. Nutr. 2006, 136, 576–581. [Google Scholar]
- Sandström, B.; Almgren, A.; Kivisto, B.; Cederblad, A. Effect of protein level and protein source on zinc absorption in humans. J. Nutr. 1989, 119, 48–53. [Google Scholar]
- Hurrell, R.F.; Juillerat, M.A.; Reddy, M.B.; Lynch, S.R.; Dassenko, S.A.; Cook, J.D. Soy protein, phytate, and iron absorption in humans. Am. J. Clin. Nutr. 1992, 56, 573–578. [Google Scholar]
- Lönnerdal, B.; Cederblad, A.; Davidsson, L.; Sandstrom, B. The effect of individual components of soy formula and cows’ milk formula on zinc bioavailability. Am. J. Clin. Nutr. 1984, 40, 1064–1070. [Google Scholar]
- Cook, J.D.; Morck, T.A.; Lynch, S.R. The inhibitory effect of soy products on nonheme iron absorption in man. Am. J. Clin. Nutr. 1981, 34, 2622–2629. [Google Scholar]
- Davidsson, L.; Almgren, A.; Sandstrom, B.; Juillerat, M.; Hurrell, R.F. Zinc absorption in adult humans: The effect of protein sources added to liquid test meals. Br. J. Nutr. 1996, 75, 607–613. [Google Scholar]
- Hallberg, L.; Brune, M.; Erlandsson, M.; Sandberg, A.; Rossander-Hulten, L. Calcium: Effect of different amounts on nonheme- and heme-iron absorption in humans. Am. J. Clin. Nutr. 1991, 53, 112–119. [Google Scholar]
- Galan, P.; Cherouvrier, F.; Preziosi, P.; Hercberg, S. Effects of the increasing consumption of dairy products upon iron absorption. Eur. J. Clin. Nutr. 1991, 45, 553–559. [Google Scholar]
- Gleerup, A.; Rossander-Hulthen, L.; Gramatkovski, E.; Hallberg, L. Iron absorption from the whole diet: Comparison of the effect of two different distributions of daily calcium intake. Am. J. Clin. Nutr. 1995, 61, 97–104. [Google Scholar]
- Reddy, M.; Cook, J. Effect of calcium intake on nonheme-iron absorption from a complete diet. Am. J. Clin. Nutr. 1997, 65, 1820–1825. [Google Scholar]
- Minihane, A.M.; Fairweather-Tait, S.J. Effect of calcium supplementation on daily nonheme-iron absorption and long-term iron status. Am. J. Clin. Nutr. 1998, 68, 96–102. [Google Scholar]
- Cook, J.D.; Dassenko, S.A.; Whittaker, P. Calcium supplementation: Effect on iron absorption. Am. J. Clin. Nutr. 1991, 53, 106–111. [Google Scholar]
- Hallberg, L.; Rossander-Hulthen, L.; Brune, M.; Gleerup, A. Inhibition of haem-iron absorption in man by calcium. Br. J. Nutr. 1993, 69, 533–540. [Google Scholar]
- Sokoll, L.J.; Dawson-Hughes, B. Calcium supplementation and plasma ferritin concentrations in premenopausal women. Am. J. Clin. Nutr. 1992, 56, 1045–1048. [Google Scholar]
- Dawson-Hughes, B.; Seligson, F.H.; Hughes, V.A. Effects of calcium carbonate and hydroxyapatite on zinc and iron retention in postmenopausal women. Am. J. Clin. Nutr. 1986, 44, 83–88. [Google Scholar]
- Spencer, H.; Kramer, L.; Norris, C.; Osis, D. Effect of calcium and phosphorus on zinc metabolism in man. Am. J. Clin. Nutr. 1984, 40, 1213–1218. [Google Scholar]
- McKenna, A.A.; Ilich, J.Z.; Andon, M.B.; Wang, C.; Matkovic, V. Zinc balance in adolescent females consuming a low- or high-calcium diet. Am. J. Clin. Nutr. 1997, 65, 1460–1464. [Google Scholar]
- Hallberg, L.; Brune, M.; Rossander, L. Effect of ascorbic acid on iron absorption from different types of meals: Studies with ascorbic-acid-rich foods and synthetic ascorbic acid given in different amounts with different meals. Hum. Nutr. Appl. Nutr. 1986, 40, 97–113. [Google Scholar]
- Diaz, M.; Rosado, J.L.; Allen, L.H.; Abrams, S.; Garcia, O.P. The efficacy of a local ascorbic acid-rich food in improving iron absorption from Mexican diets: A field study using stable isotopes. Am. J. Clin. Nutr. 2003, 78, 436–440. [Google Scholar]
- Cook, J.D.; Reddy, M.B. Effect of ascorbic acid intake on nonheme-iron absorption from a complete diet. Am. J. Clin. Nutr. 2001, 73, 93–98. [Google Scholar]
- Beck, K.; Conlon, C.A.; Kruger, R.; Coad, J.; Stonehouse, W. Gold kiwifruit consumed with an iron-fortified breakfast cereal meal improves iron status in women with low iron stores: A 16-week randomised controlled trial. Br. J. Nutr. 2011, 105, 101–109. [Google Scholar]
- Garcia, O.P.; Diaz, M.; Rosado, J.L.; Allen, L.H. Ascorbic acid from lime juice does not improve the iron status of iron-deficient women in rural Mexico. Am. J. Clin. Nutr. 2003, 78, 267–273. [Google Scholar]
- Gillooly, M.; Bothwell, T.H.; Torrance, J.D.; MacPhail, A.P.; Derman, D.P.; Bezwoda, W.R.; Mills, W.; Charlton, R.W.; Mayet, F. The effects of organic acids, phytates and polyphenols on the absorption of iron from vegetables. Br. J. Nutr. 1983, 49, 331–342. [Google Scholar]
- Sandström, B.; Cederblad, A. Effect of ascorbic acid on the absorption of zinc and calcium in man. Int. J. Vitam. Nutr. Res. 1987, 57, 87–90. [Google Scholar]
- Nemeth, E.; Ganz, T. Regulation of iron metabolism by hepcidin. Annu. Rev. Nutr. 2006, 26, 323–342. [Google Scholar] [CrossRef]
- Brown, K.H.; Rivera, J.A.; Bhutta, Z.; Gibson, R.S.; King, J.C.; Lonnerdal, B.; Ruel, M.T.; Sandstrom, B.; Wasantwisut, E.; Hotz, C. International Zinc Nutrition Consultative Group (IZiNCG) technical document #1. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr. Bull. 2004, 25, S99–S203. [Google Scholar]
- Sian, L.; Mingyan, X.; Miller, L.V.; Tong, L.; Krebs, N.F.; Hambidge, K.M. Zinc absorption and intestinal losses of endogenous zinc in young Chinese women with marginal zinc intakes. Am. J. Clin. Nutr. 1996, 63, 348–353. [Google Scholar]
- Cooper, M.; Greene-Finestone, L.; Lowell, H.; Levesque, J.; Robinson, S. Iron Sufficiency of Canadians; Minister of Industry: Ottawa, Canada, 2012. [Google Scholar]
- UK Department of Health. National Diet and Nutrition Survey: Headline Results from Years 1 and 2 (combined) of the Rolling Programme, 2008/09–2009/10; Supplementary Report: Blood Analytes, 2011. Available online: https://www.gov.uk/government/publications/national-diet-and-nutrition-survey-headline-results-from-years-1-and-2-combined-of-the-rolling-programme-2008-09–2009-10-supplementary-report-blood-analytes (accessed on 27 April 2013).
- US Centers for Disease Control and Prevention. Second National Report on Biochemical Indicators of Diet and Nutrition in the US Population 2012, 2012. Available online: http://www.cdc.gov/nutritionreport/report.html (accessed 29 April 2013).
- Lowe, N.M.; Fekete, K.; Decsi, T. Methods of assessment of zinc status in humans: A systematic review. Am. J. Clin. Nutr. 2009, 89, 2040S–2051S. [Google Scholar]
- Hess, S.Y.; Peerson, J.M.; King, J.C.; Brown, K.H. Use of serum zinc concentration as an indicator of population zinc status. Food Nutr. Bull. 2007, 28, S403–S429. [Google Scholar]
- Gibson, R.S.; Hess, S.Y.; Hotz, C.; Brown, K.H. Indicators of zinc status at the population level: A review of the evidence. Br. J. Nutr. 2008, 99, S14–S23. [Google Scholar]
- Hotz, C.; Brown, K.H. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr. Bull. 2004, 25, S94–S203. [Google Scholar]
- Hotz, C.; Peerson, J.M.; Brown, K.H. Suggested lower cutoffs of serum zinc concentrations for assessing zinc status: Reanalysis of the second National Health and Nutrition Examination Survey data (1976–1980). Am. J. Clin. Nutr. 2003, 78, 756–764. [Google Scholar]
- Yokoi, K.; Sandstead, H.H.; Egger, N.G.; Alcock, N.W.; Sadagopa Ramanujam, V.M.; Dayal, H.H.; Penland, J.G. Association between zinc pool sizes and iron stores in premenopausal women without anaemia. Br. J. Nutr. 2007, 98, 1214–1223. [Google Scholar]
- Cole, C.R.; Grant, F.K.; Swaby-Ellis, E.D.; Smith, J.L.; Jacques, A.; Northrop-Clewes, C.A.; Caldwell, K.L.; Pfeiffer, C.M.; Ziegler, T.R. Zinc and iron deficiency and their interrelations in low-income African American and Hispanic children in Atlanta. Am. J. Clin. Nutr. 2010, 91, 1027–1034. [Google Scholar]
- Hettiarachchi, M.; Liyanage, C.; Wickremasinghe, R.; Hilmers, D.C.; Abrahams, S.A. Prevalence and severity of micronutrient deficiency: A cross-sectional study among adolescents in Sri Lanka. Asia Pac. J. Clin. Nutr. 2006, 15, 56–63. [Google Scholar]
- Yokoi, K.; Alcock, N.W.; Sandstead, H.H. Iron and zinc nutriture of premenopausal women: Associations of diet with serum ferritin and plasma zinc disappearance and of serum ferritin with plasma zinc and plasma zinc disappearance. J. Lab. Clin. Med. 1994, 124, 852–861. [Google Scholar]
- Solomons, N.W. Competitive interaction of iron and zinc in the diet: Consequences for human nutrition. J. Nutr. 1986, 116, 927–935. [Google Scholar]
- Olivares, M.; Pizarro, F.; Ruz, M.; Lopez de Romana, D. Acute inhibition of iron bioavailability by zinc: Studies in humans. Biometals 2012, 25, 657–664. [Google Scholar] [CrossRef]
- Solomons, N.W.; Jacob, R.A. Studies on the bioavailability of zinc in humans: Effects of heme and nonheme iron on the absorption of zinc. Am. J. Clin. Nutr. 1981, 34, 475–482. [Google Scholar]
- Solomons, N.W.; Pineda, O.; Viteri, F.; Sandstead, H.H. Studies on the bioavailability of zinc in humans: Mechanism of the intestinal interaction of nonheme iron and zinc. J. Nutr. 1983, 113, 337–349. [Google Scholar]
- Meadows, N.J.; Grainger, S.L.; Ruse, W.; Keeling, P.W.; Thompson, R.P. Oral iron and the bioavailability of zinc. Br. Med. J. (Clin. Res. Ed.). 1983, 287, 1013–1014. [Google Scholar]
- Solomons, N.W. Dietary sources of zinc and factors affecting its bioavailability. Food Nutr. Bull. 2001, 22, 138–154. [Google Scholar]
- Dijkhuizen, M.A.; Wieringa, F.T.; West, C.E.; Martuti, S. Muhilal effects of iron and zinc supplementation in Indonesian infants on micronutrient status and growth. J. Nutr. 2001, 131, 2860–2865. [Google Scholar]
- Sheldon, W.L.; Aspillaga, M.O.; Smith, P.A.; Lind, T. The effects of oral iron supplementation on zinc and magnesium levels during pregnancy. Br. J. Obstet. Gynaecol. 1985, 92, 892–898. [Google Scholar] [CrossRef]
- Yip, R.; Reeves, J.D.; Lonnerdal, B.; Keen, C.L.; Dallman, P.R. Does iron supplementation compromise zinc nutrition in healthy infants? Am. J. Clin. Nutr. 1985, 42, 683–687. [Google Scholar]
- Friel, J.K.; Aziz, K.; Andrews, W.L.; Harding, S.V.; Courage, M.L.; Adams, R.J. A double-masked, randomized control trial of iron supplementation in early infancy in healthy term breast-fed infants. J. Pediatr. 2003, 143, 582–586. [Google Scholar]
- Makrides, M.; Crowther, C.A.; Gibson, R.A.; Gibson, R.S.; Skeaff, C.M. Efficacy and tolerability of low-dose iron supplements during pregnancy: A randomized controlled trial. Am. J. Clin. Nutr. 2003, 78, 145–153. [Google Scholar]
- Dawson, E.B.; Albers, J.; McGanity, W.J. Serum zinc changes due to iron supplementation in teen-age pregnancy. Am. J. Clin. Nutr. 1989, 50, 848–852. [Google Scholar]
- Prosser, N.R.; Heath, A.L.; Williams, S.M.; Gibson, R.S. Influence of an iron intervention on the zinc status of young adult New Zealand women with mild iron deficiency. Br. J. Nutr. 2010, 104, 742–750. [Google Scholar] [CrossRef]
- Whittaker, P. Iron and zinc interactions in humans. Am. J. Clin. Nutr. 1998, 68, 442S–446S. [Google Scholar]
- Fairweather-Tait, S.J. Iron-zinc and calcium-Fe interactions in relation to Zn and Fe absorption. Proc. Nutr. Soc. 1995, 54, 465–473. [Google Scholar] [CrossRef]
- Valberg, L.S.; Flanagan, P.R.; Chamberlain, M.J. Effects of iron, tin, and copper on zinc absorption in humans. Am. J. Clin. Nutr. 1984, 40, 536–541. [Google Scholar]
- Sandström, B.; Davidsson, L.; Cederblad, A.; Lonnerdal, B. Oral iron, dietary ligands and zinc absorption. J. Nutr. 1985, 115, 411–414. [Google Scholar]
- Davidsson, L.; Almgren, A.; Sandstrom, B.; Hurrell, R.F. Zinc absorption in adult humans: The effect of iron fortification. Br. J. Nutr. 1995, 74, 417–425. [Google Scholar] [CrossRef]
- Donangelo, C.M.; Woodhouse, L.R.; King, S.M.; Viteri, F.E.; King, J.C. Supplemental zinc lowers measures of iron status in young women with low iron reserves. J. Nutr. 2002, 132, 1860–1864. [Google Scholar]
- Yadrick, M.K.; Kenney, M.A.; Winterfeldt, E.A. Iron, copper, and zinc status: Response to supplementation with zinc or zinc and iron in adult females. Am. J. Clin. Nutr. 1989, 49, 145–150. [Google Scholar]
- Fahmida, U.; Rumawas, J.S.; Utomo, B.; Patmonodewo, S.; Schultink, W. Zinc-iron, but not zinc-alone supplementation, increased linear growth of stunted infants with low haemoglobin. Asia Pac. J. Clin. Nutr. 2007, 16, 301–309. [Google Scholar]
- Wuehler, S.E.; Sempertegui, F.; Brown, K.H. Dose-response trial of prophylactic zinc supplements, with or without copper, in young Ecuadorian children at risk of zinc deficiency. Am. J. Clin. Nutr. 2008, 87, 723–733. [Google Scholar]
- Rosado, J.L.; Diaz, M.; Gonzalez, K.; Griffin, I.; Abrams, S.A.; Preciado, R. The addition of milk or yogurt to a plant-based diet increases zinc bioavailability but does not affect iron bioavailability in women. J. Nutr. 2005, 135, 465–468. [Google Scholar]
- Penny, M.E.; Marin, R.M.; Duran, A.; Peerson, J.M.; Lanata, C.F.; Lonnerdal, B.; Black, R.E.; Brown, K.H. Randomized controlled trial of the effect of daily supplementation with zinc or multiple micronutrients on the morbidity, growth, and micronutrient status of young Peruvian children. Am. J. Clin. Nutr. 2004, 79, 457–465. [Google Scholar]
- Brown, K.H.; Peerson, J.M.; Baker, S.K.; Hess, S.Y. Preventive zinc supplementation among infants, preschoolers, and older prepubertal children. Food Nutr. Bull. 2009, 30, S12–S40. [Google Scholar]
- Friel, J.K.; Serfass, R.E.; Fennessey, P.V.; Miller, L.V.; Andrews, W.L.; Simmons, B.S.; Downton, G.F.; Kwa, P.G. Elevated intakes of zinc in infant formulas do not interfere with iron absorption in premature infants. J. Pediatr. Gastroenterol. Nutr. 1998, 27, 312–316. [Google Scholar] [CrossRef]
- Rossander-Hulten, L.; Brune, M.; Sandstrom, B.; Lonnerdal, B.; Hallberg, L. Competitive inhibition of iron absorption by manganese and zinc in humans. Am. J. Clin. Nutr. 1991, 54, 152–156. [Google Scholar]
- Olivares, M.; Pizarro, F.; Ruz, M. Zinc inhibits nonheme iron bioavailability in humans. Biol. Trace Elem. Res. 2007, 117, 7–14. [Google Scholar] [CrossRef]
- Lopez de Romana, D.; Ruz, M.; Pizarro, F.; Landeta, L.; Olivares, M.A. Supplementation with zinc between meals has no effect on subsequent iron absorption or on iron status of Chilean women. Nutrition 2008, 24, 957–963. [Google Scholar] [CrossRef]
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