Nutrients 2013, 5(7), 2372-2383; doi:10.3390/nu5072372
Article

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

Received: 4 March 2013; in revised form: 9 June 2013 / Accepted: 14 June 2013 / Published: 1 July 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Impaired mitochondrial capacity may be implicated in the pathology of chronic metabolic diseases. To elucidate the effect of ethyl pyruvate supplementation on skeletal muscles metabolism we examined changes in activities of mitochondrial and antioxidant enzymes, as well as sulfhydryl groups oxidation (an indirect marker of oxidative stress) during the development of obesity. After 6 weeks feeding of control or high fat diet, Wistar rats were divided into four groups: control diet, control diet and ethyl pyruvate, high fat diet, and high fat diet and ethyl pyruvate. Ethyl pyruvate was administered as 0.3% solution in drinking water, for the following 6 weeks. High fat diet feeding induced the increase of activities 3-hydroxyacylCoA dehydrogenase, citrate synthase, and fumarase. Moreover, higher catalase and superoxide dismutase activities, as well as sulfhydryl groups oxidation, were noted. Ethyl pyruvate supplementation did not affect the mitochondrial enzymes’ activities, but induced superoxide dismutase activity and sulfhydryl groups oxidation. All of the changes were observed in soleus muscle, but not in extensor digitorum longus muscle. Additionally, positive correlations between fasting blood insulin concentration and activities of catalase (p = 0.04), and superoxide dismutase (p = 0.01) in soleus muscle were noticed. Prolonged ethyl pyruvate consumption elevated insulin concentration, which may cause modifications in oxidative type skeletal muscles.
Keywords: mitochondrial metabolism; skeletal muscle; sulfhydryl groups; insulin; obesity; diet
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MDPI and ACS Style

Olek, R.A.; Ziolkowski, W.; Wierzba, T.H.; Kaczor, J.J. Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet. Nutrients 2013, 5, 2372-2383.

AMA Style

Olek RA, Ziolkowski W, Wierzba TH, Kaczor JJ. Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet. Nutrients. 2013; 5(7):2372-2383.

Chicago/Turabian Style

Olek, Robert A.; Ziolkowski, Wieslaw; Wierzba, Tomasz H.; Kaczor, Jan J. 2013. "Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet." Nutrients 5, no. 7: 2372-2383.

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