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Nutrients 2013, 5(5), 1660-1671; doi:10.3390/nu5051660
Article

Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells

1
, 2
 and 1,3,*
Received: 18 April 2013; in revised form: 6 May 2013 / Accepted: 8 May 2013 / Published: 21 May 2013
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Abstract: Uncoupling protein 3 (UCP3) is a mitochondrial membrane transporter that is expressed mainly in skeletal muscle where it plays an important role in energy expenditure and fat oxidation. In this study, we investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on UCP3 gene expression in C2C12 muscle cells. EPA and DHA up-regulated UCP3 mRNA level in a dose-dependent manner and similarly increased UCP3 promoter activity in C2C12 muscle cells. To determine whether AMP-activated protein kinase (AMPK) signaling may also directly regulate UCP3 expression, 5′-amino-4-imidazolecarboxamide-ribonucleoside (AICAR), an AMP analog that activates AMPK, was treated in C2C12 muscle cells. AICAR showed additive effects with EPA or DHA on the UCP3 promoter activation. These results indicate that EPA and DHA directly regulate the gene expression of UCP3, potentially through AMPK-mediated pathway in C2C12 muscle cells.
Keywords: eicosapentaenoic acid; docosahexaenoic acid; uncoupling protein 3; AMP-activated protein kinase eicosapentaenoic acid; docosahexaenoic acid; uncoupling protein 3; AMP-activated protein kinase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lee, M.-S.; Kim, I.-H.; Kim, Y. Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells. Nutrients 2013, 5, 1660-1671.

AMA Style

Lee M-S, Kim I-H, Kim Y. Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells. Nutrients. 2013; 5(5):1660-1671.

Chicago/Turabian Style

Lee, Mak-Soon; Kim, In-Hwan; Kim, Yangha. 2013. "Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells." Nutrients 5, no. 5: 1660-1671.


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