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Deficiency in the 15 kDa Selenoprotein Inhibits Human Colon Cancer Cell Growth
Cancer Prevention Fellowship Program, National Cancer Institute, Rockville, MD 20892, USA
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, Bethesda, MD 20892, USA
Nutritional Science Research Group, National Cancer Institute, Rockville, MD 20892, USA
Department of Biological Sciences, Towson University, Towson, MD 21252, USA
* Author to whom correspondence should be addressed.
Received: 1 August 2011; in revised form: 15 August 2011 / Accepted: 29 August 2011 / Published: 5 September 2011
Abstract: Selenium is an essential micronutrient for humans and animals, and is thought to provide protection against some forms of cancer. These protective effects appear to be mediated, at least in part, through selenium-containing proteins (selenoproteins). Recent studies in a mouse colon cancer cell line have shown that the 15 kDa selenoprotein (Sep15) may also play a role in promoting colon cancer. The current study investigated whether the effects of reversing the cancer phenotype observed when Sep15 was removed in mouse colon cancer cells, were recapitulated in HCT116 and HT29 human colorectal carcinoma cells. Targeted down-regulation of Sep15 using RNAi technology in these human colon cancer cell lines resulted in similarly decreased growth under anchorage-dependent and anchorage-independent conditions. However, the magnitude of reduction in cell growth was much less than in the mouse colon cancer cell line investigated previously. Furthermore, changes in cell cycle distribution were observed, indicating a delayed release of Sep15 deficient cells from the G0/G1 phase after synchronization. The potential mechanism by which human colon cancer cells lacking Sep15 revert their cancer phenotype will need to be explored further.
Keywords: HCT116 cells; HT29 cells; shRNA; selenium; cancer prevention
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Tsuji, P.A.; Naranjo-Suarez, S.; Carlson, B.A.; Tobe, R.; Yoo, M.-H.; Davis, C.D. Deficiency in the 15 kDa Selenoprotein Inhibits Human Colon Cancer Cell Growth. Nutrients 2011, 3, 805-817.
Tsuji PA, Naranjo-Suarez S, Carlson BA, Tobe R, Yoo M-H, Davis CD. Deficiency in the 15 kDa Selenoprotein Inhibits Human Colon Cancer Cell Growth. Nutrients. 2011; 3(9):805-817.
Tsuji, Petra A.; Naranjo-Suarez, Salvador; Carlson, Bradley A.; Tobe, Ryuta; Yoo, Min-Hyuk; Davis, Cindy D. 2011. "Deficiency in the 15 kDa Selenoprotein Inhibits Human Colon Cancer Cell Growth." Nutrients 3, no. 9: 805-817.