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Nutrients 2011, 3(11), 987-1002; doi:10.3390/nu3110987
Article

Hepatic Oxidative Stress in Fructose-Induced Fatty Liver Is Not Caused by Sulfur Amino Acid Insufficiency

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Received: 19 September 2011; in revised form: 24 October 2011 / Accepted: 4 November 2011 / Published: 18 November 2011
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Abstract: Fructose-sweetened liquid consumption is associated with fatty liver and oxidative stress. In rodent models of fructose-mediated fatty liver, protein consumption is decreased. Additionally, decreased sulfur amino acid intake is known to cause oxidative stress. Studies were designed to test whether oxidative stress in fructose-sweetened liquid-induced fatty liver is caused by decreased ad libitum solid food intake with associated inadequate sulfur amino acid intake. C57BL6 mice were grouped as: control (ad libitum water), fructose (ad libitum 30% fructose-sweetened liquid), glucose (ad libitum 30% glucose-sweetened water) and pair-fed (ad libitum water and sulfur amino acid intake same as the fructose group). Hepatic and plasma thiol-disulfide antioxidant status were analyzed after five weeks. Fructose- and glucose-fed mice developed fatty liver. The mitochondrial antioxidant protein, thioredoxin-2, displayed decreased abundance in the liver of fructose and glucose-fed mice compared to controls. Glutathione/glutathione disulfide redox potential (EhGSSG) and abundance of the cytoplasmic antioxidant protein, peroxiredoxin-2, were similar among groups. We conclude that both fructose and glucose-sweetened liquid consumption results in fatty liver and upregulated thioredoxin-2 expression, consistent with mitochondrial oxidative stress; however, inadequate sulfur amino acid intake was not the cause of this oxidative stress.
Keywords: cystine; methionine; thioredoxin; redox potential; mitochondria; obesity cystine; methionine; thioredoxin; redox potential; mitochondria; obesity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kunde, S.S.; Roede, J.R.; Vos, M.B.; Orr, M.L.; Go, Y.-M.; Park, Y.; Ziegler, T.R.; Jones, D.P. Hepatic Oxidative Stress in Fructose-Induced Fatty Liver Is Not Caused by Sulfur Amino Acid Insufficiency. Nutrients 2011, 3, 987-1002.

AMA Style

Kunde SS, Roede JR, Vos MB, Orr ML, Go Y-M, Park Y, Ziegler TR, Jones DP. Hepatic Oxidative Stress in Fructose-Induced Fatty Liver Is Not Caused by Sulfur Amino Acid Insufficiency. Nutrients. 2011; 3(11):987-1002.

Chicago/Turabian Style

Kunde, Sachin S.; Roede, James R.; Vos, Miriam B.; Orr, Michael L.; Go, Young-Mi; Park, Youngja; Ziegler, Thomas R.; Jones, Dean P. 2011. "Hepatic Oxidative Stress in Fructose-Induced Fatty Liver Is Not Caused by Sulfur Amino Acid Insufficiency." Nutrients 3, no. 11: 987-1002.


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