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Nutrients 2018, 10(2), 144; https://doi.org/10.3390/nu10020144

Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch

1
Academic Institute, College of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
2
Department of Nutritional Sciences, Faculty of Health and Welfare Science, Nayoro City University, Kita 8-1, Nishi 4, Nayoro, Hokkaido 096-8641, Japan
3
Department of Agricultural and Life Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan
*
Author to whom correspondence should be addressed.
Received: 6 December 2017 / Revised: 13 January 2018 / Accepted: 26 January 2018 / Published: 29 January 2018
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Abstract

The hydrogen molecule (H2), which has low redox potential, is produced by colonic fermentation. We examined whether increased H2 concentration in the portal vein in rats fed high amylose maize starch (HAS) helped alleviate oxidative stress, and whether the transplantation of rat colonic microbiota with high H2 production can shift low H2-generating rats (LG) to high H2-generating rats (HG). Rats were fed a 20% HAS diet for 10 days and 13 days in experiments 1 and 2, respectively. After 10 days (experiment 1), rats underwent a hepatic ischemia–reperfusion (IR) operation. Rats were then categorized into quintiles of portal H2 concentration. Plasma alanine aminotransferase activity and hepatic oxidized glutathione concentration were significantly lower as portal H2 concentration increased. In experiment 2, microbiota derived from HG (the transplantation group) or saline (the control group) were orally inoculated into LG on days 3 and 4. On day 13, portal H2 concentration in the transplantation group was significantly higher compared with the control group, and positively correlated with genera Bifidobacterium, Allobaculum, and Parabacteroides, and negatively correlated with genera Bacteroides, Ruminococcus, and Escherichia. In conclusion, the transplantation of microbiota derived from HG leads to stable, high H2 production in LG, with the resultant high production of H2 contributing to the alleviation of oxidative stress. View Full-Text
Keywords: hydrogen; resistant starch; microbiota transplantation; antioxidant effect; rats hydrogen; resistant starch; microbiota transplantation; antioxidant effect; rats
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nishimura, N.; Tanabe, H.; Komori, E.; Sasaki, Y.; Inoue, R.; Yamamoto, T. Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch. Nutrients 2018, 10, 144.

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