Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Nutrients 2009, 1(2), 133-147; doi:10.3390/nu1020133
Article

Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1

1
, 1
, 1, 1, 1,2
 and 1,*
Received: 7 October 2009; Accepted: 28 October 2009 / Published: 29 October 2009
(This article belongs to the Special Issue Foodomics 2009)
View Full-Text   |   Download PDF [460 KB, uploaded 29 October 2009]
Abstract: Polyunsaturated fatty acids affect gene expression mainly through peroxisome proliferator-activated receptors (PPARs) and sterol regulatory element binding proteins (SREBPs), but how monounsaturated fatty acids affect gene expression is poorly understood. In HepG2 cells, oleate supplementation has been shown to increase secretion of hepatic lipase (HL). We hypothesized that oleate affects HL gene expression at the transcriptional level. To test this, we studied the effect of oleate on HL promoter activity using HepG2 cells and the proximal HL promoter region (700 bp). Oleate increased HL expression and promoter activity 1.3–2.1 fold and reduced SREBP activity by 50%. Downregulation of SREBP activity by incubation with cholesterol+25-hydroxycholesterol had no effect on HL promoter activity. Overexpression of SREBP2, but not SREBP1, reduced HL promoter activity, which was effected mainly through the USF1 binding site at -307/-312. Oleate increased the nuclear abundance of USF1 protein 2.7 ± 0.6 fold, while USF1 levels were reduced by SREBP2 overexpression. We conclude that oleate increases HL gene expression via USF1. USF1 may be an additional fatty acid sensor in liver cells.
Keywords: monounsaturated fatty acids; sterol-responsive element binding protein SREBP2; upstream stimulatory factor USF1; LIPC; transcriptional regulation monounsaturated fatty acids; sterol-responsive element binding protein SREBP2; upstream stimulatory factor USF1; LIPC; transcriptional regulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Van Deursen, D.; Van Leeuwen, M.; Akdogan, D.; Adams, H.; Jansen, H.; Verhoeven, A.J.M. Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1. Nutrients 2009, 1, 133-147.

AMA Style

Van Deursen D, Van Leeuwen M, Akdogan D, Adams H, Jansen H, Verhoeven AJM. Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1. Nutrients. 2009; 1(2):133-147.

Chicago/Turabian Style

Van Deursen, Diederik; Van Leeuwen, Marije; Akdogan, Deniz; Adams, Hadie; Jansen, Hans; Verhoeven, Adrie J. M. 2009. "Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1." Nutrients 1, no. 2: 133-147.


Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert