Microscopic Characterization of the Brazilian Giant Samba Virus
AbstractPrior to the discovery of the mimivirus in 2003, viruses were thought to be physically small and genetically simple. Mimivirus, with its ~750-nm particle size and its ~1.2-Mbp genome, shattered these notions and changed what it meant to be a virus. Since this discovery, the isolation and characterization of giant viruses has exploded. One of the more recently discovered giant viruses, Samba virus, is a Mimivirus that was isolated from the Rio Negro in the Brazilian Amazon. Initial characterization of Samba has revealed some structural information, although the preparation techniques used are prone to the generation of structural artifacts. To generate more native-like structural information for Samba, we analyzed the virus through cryo-electron microscopy, cryo-electron tomography, scanning electron microscopy, and fluorescence microscopy. These microscopy techniques demonstrated that Samba particles have a capsid diameter of ~527 nm and a fiber length of ~155 nm, making Samba the largest Mimivirus yet characterized. We also compared Samba to a fiberless mimivirus variant. Samba particles, unlike those of mimivirus, do not appear to be rigid, and quasi-icosahedral, although the two viruses share many common features, including a multi-layered capsid and an asymmetric nucleocapsid, which may be common amongst the Mimiviruses. View Full-Text
- Supplementary File 1:
MP4-Document (MP4, 16682 KB)
Share & Cite This Article
Schrad, J.R.; Young, E.J.; Abrahão, J.S.; Cortines, J.R.; Parent, K.N. Microscopic Characterization of the Brazilian Giant Samba Virus. Viruses 2017, 9, 30.
Schrad JR, Young EJ, Abrahão JS, Cortines JR, Parent KN. Microscopic Characterization of the Brazilian Giant Samba Virus. Viruses. 2017; 9(2):30.Chicago/Turabian Style
Schrad, Jason R.; Young, Eric J.; Abrahão, Jônatas S.; Cortines, Juliana R.; Parent, Kristin N. 2017. "Microscopic Characterization of the Brazilian Giant Samba Virus." Viruses 9, no. 2: 30.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.