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Viruses 2017, 9(2), 30; doi:10.3390/v9020030

Microscopic Characterization of the Brazilian Giant Samba Virus

1
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, 48824 MI, USA
2
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil
3
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille University, 13385 Marseille Cedex 05, France
4
Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editor: Bernard La Scola
Received: 18 November 2016 / Revised: 25 January 2017 / Accepted: 2 February 2017 / Published: 14 February 2017
(This article belongs to the Special Issue Viruses of Protozoa)
View Full-Text   |   Download PDF [61439 KB, uploaded 16 February 2017]   |  

Abstract

Prior to the discovery of the mimivirus in 2003, viruses were thought to be physically small and genetically simple. Mimivirus, with its ~750-nm particle size and its ~1.2-Mbp genome, shattered these notions and changed what it meant to be a virus. Since this discovery, the isolation and characterization of giant viruses has exploded. One of the more recently discovered giant viruses, Samba virus, is a Mimivirus that was isolated from the Rio Negro in the Brazilian Amazon. Initial characterization of Samba has revealed some structural information, although the preparation techniques used are prone to the generation of structural artifacts. To generate more native-like structural information for Samba, we analyzed the virus through cryo-electron microscopy, cryo-electron tomography, scanning electron microscopy, and fluorescence microscopy. These microscopy techniques demonstrated that Samba particles have a capsid diameter of ~527 nm and a fiber length of ~155 nm, making Samba the largest Mimivirus yet characterized. We also compared Samba to a fiberless mimivirus variant. Samba particles, unlike those of mimivirus, do not appear to be rigid, and quasi-icosahedral, although the two viruses share many common features, including a multi-layered capsid and an asymmetric nucleocapsid, which may be common amongst the Mimiviruses. View Full-Text
Keywords: Mimiviridae; cryo-electron microscopy; cryo-electron tomography; fluorescence microscopy; scanning electron microscopy; giant viruses; nucleocytoplasmic large DNA virus (NCLDV); Megavirales; mimivirus Mimiviridae; cryo-electron microscopy; cryo-electron tomography; fluorescence microscopy; scanning electron microscopy; giant viruses; nucleocytoplasmic large DNA virus (NCLDV); Megavirales; mimivirus
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MDPI and ACS Style

Schrad, J.R.; Young, E.J.; Abrahão, J.S.; Cortines, J.R.; Parent, K.N. Microscopic Characterization of the Brazilian Giant Samba Virus. Viruses 2017, 9, 30.

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