Next Article in Journal
Inoculation of Goats, Sheep, and Horses with MERS-CoV Does Not Result in Productive Viral Shedding
Next Article in Special Issue
Quantification of HEV RNA by Droplet Digital PCR
Previous Article in Journal / Special Issue
Role of Envelopment in the HEV Life Cycle
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(8), 227; doi:10.3390/v8080227

A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus

1
Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA
2
Nanjing Red Cross Blood Center, Nanjing 210003, Jiangsu, China
3
College of Veterinary Medicine, Northwest A&F University, Yangling, Xi’an 712100, Shaanxi, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jacques Izopet
Received: 23 June 2016 / Revised: 28 July 2016 / Accepted: 9 August 2016 / Published: 18 August 2016
(This article belongs to the Special Issue Recent Progress in Hepatitis E Virus Research)
View Full-Text   |   Download PDF [1586 KB, uploaded 19 August 2016]   |  

Abstract

Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. View Full-Text
Keywords: hepatitis E virus; HEV; ORF3; VP13; PXXP motif; linear epitope; monoclonal antibody hepatitis E virus; HEV; ORF3; VP13; PXXP motif; linear epitope; monoclonal antibody
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yang, Y.; Lin, S.; Nan, Y.; Ma, Z.; Yang, L.; Zhang, Y. A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus. Viruses 2016, 8, 227.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top