Next Article in Journal
Mouse Systems to Model Hepatitis C Virus Treatment and Associated Resistance
Previous Article in Journal
The Feat of Packaging Eight Unique Genome Segments
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(6), 173; doi:10.3390/v8060173

PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells

Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep., 0033 Oslo, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 22 April 2016 / Revised: 31 May 2016 / Accepted: 3 June 2016 / Published: 21 June 2016
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [2891 KB, uploaded 21 June 2016]   |  

Abstract

The double-stranded RNA-activated protein kinase R (PKR) is a Type I interferon (IFN) stimulated gene that has important biological and immunological functions. In viral infections, in general, PKR inhibits or promotes viral replication, but PKR-IPNV interaction has not been previously studied. We investigated the involvement of PKR during infectious pancreatic necrosis virus (IPNV) infection using a custom-made rabbit antiserum and the PKR inhibitor C16. Reactivity of the antiserum to PKR in CHSE-214 cells was confirmed after IFNα treatment giving an increased protein level. IPNV infection alone did not give increased PKR levels by Western blot, while pre-treatment with PKR inhibitor before IPNV infection gave decreased eukaryotic initiation factor 2-alpha (eIF2α) phosphorylation. This suggests that PKR, despite not being upregulated, is involved in eIF2α phosphorylation during IPNV infection. PKR inhibitor pre-treatment resulted in decreased virus titers, extra- and intracellularly, concomitant with reduction of cells with compromised membranes in IPNV-permissive cell lines. These findings suggest that IPNV uses PKR activation to promote virus replication in infected cells. View Full-Text
Keywords: PKR; cloning; PKR inhibitor; IPNV; eIF2α phosphorylation; necrosis PKR; cloning; PKR inhibitor; IPNV; eIF2α phosphorylation; necrosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gamil, A.A.; Xu, C.; Mutoloki, S.; Evensen, Ø. PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells. Viruses 2016, 8, 173.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top