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Viruses 2016, 8(6), 159; doi:10.3390/v8060159

Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies

1
Research Institute for Biological Safety Problems, RK ME&S – Science Committee, Gvardeiskiy 080409, Kazakhstan
2
M. A. Aitkhozhin’s Institute of Molecular Biology and Biochemistry, RK ME&S – Science Committee, Almaty 050012, Kazakhstan
3
United States Department of Agriculture, Agricultural Research Service, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 9 April 2016 / Revised: 24 May 2016 / Accepted: 30 May 2016 / Published: 7 June 2016
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [3720 KB, uploaded 7 June 2016]   |  

Abstract

The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. View Full-Text
Keywords: sheep pox virus; vaccinia virus; recombinant protein; prokaryotic expression; neutralizing antibodies; immunogenicity sheep pox virus; vaccinia virus; recombinant protein; prokaryotic expression; neutralizing antibodies; immunogenicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chervyakova, O.V.; Zaitsev, V.L.; Iskakov, B.K.; Tailakova, E.T.; Strochkov, V.M.; Sultankulova, K.T.; Sandybayev, N.T.; Stanbekova, G.E.; Beisenov, D.K.; Abduraimov, Y.O.; Mambetaliyev, M.; Sansyzbay, A.R.; Kovalskaya, N.Y.; Nemchinov, L.G.; Hammond, R.W. Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies. Viruses 2016, 8, 159.

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