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Viruses 2016, 8(10), 268; doi:10.3390/v8100268

Molecular Characterization of a Trisegmented Mycovirus from the Plant Pathogenic Fungus Colletotrichum gloeosporioides

1
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China
2
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 21 August 2016 / Revised: 15 September 2016 / Accepted: 19 September 2016 / Published: 27 September 2016
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

A novel double-stranded RNA (dsRNA) mycovirus, consisting of three dsRNA genome segments and possibly belonging to the family Chrysoviridae, was isolated from the filamentous phytopathogenic fungus Colletotrichum gloeosporioides and designated as Colletotrichum gloeosprioides chrysovirus 1 (CgCV1). The three dsRNAs of the CgCV1 genome with lengths of 3397, 2869, and 2630 bp (dsRNAs1–3) were found to contain a single open reading frame (ORF) putatively encoding the RNA-dependent RNA polymerase (RdRp), a capsid protein, and a protease, respectively, all of which exhibited some degree of sequence similarity to the comparable putative proteins encoded by the genus Chrysovirus. The 5′- and 3′-untranslated regions in each dsRNA segment contained similar sequences that were strictly conserved at the termini. Moreover, isometric virus-like particles (VLPs) with a diameter of approximately 40 nm were extracted from fungal mycelia. Phylogenetic analysis based on the conserved dsRNA1-encoded RdRp showed that CgCV1 is a new virus belonging to the Chrysoviridae family. BLAST analysis revealed the presence of CgCV1-like sequences in the chromosomes of Medicago truncatula and Solanum tuberosum. Moreover, some sequences in the transcriptome shotgun assembly (TSA) library and expressed sequence tag database (ESTdb) of other eudicot and monocot plants were also found to be related to CgCV1. View Full-Text
Keywords: mycovirus; Chrysoviridae; Colletotrichum gloeosporioides; dsRNA virus mycovirus; Chrysoviridae; Colletotrichum gloeosporioides; dsRNA virus
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MDPI and ACS Style

Zhong, J.; Pang, X.D.; Zhu, H.J.; Gao, B.D.; Huang, W.K.; Zhou, Q. Molecular Characterization of a Trisegmented Mycovirus from the Plant Pathogenic Fungus Colletotrichum gloeosporioides. Viruses 2016, 8, 268.

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